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Desmoglein 3 antibody [MSVA-543M] HistoMAX™

Orthogonal HistoMAX
Anti-Desmoglein 3 antibody [MSVA-543M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04468
Anti-Desmoglein 3 antibody [MSVA-543M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04468
Anti-Desmoglein 3 antibody [MSVA-543M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04468

Cat. No. GTX04468

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MSVA-543M

Isotype

 IgG1

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($629),
100 μl ($299)
See all Desmoglein 3 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:75-1:150

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

Tonsil: A moderate to strong desmoglein 3 immunostaining should be seen in a fraction of squamous epithelial cells.

Negative Control

Tonsil: Desmoglein 3 immunostaining should be absent in all non-squamous epithelial cell types.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 379-491) human DSG3 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

desmoglein 3 , CDHF6 , PVA

Cellular Localization

Cell membrane , Cell junction, desmosome

Background

This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Desmoglein 3 antibody [MSVA-543M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04468

GTX04468 IHC-P Image

IHC-P analysis of human penis tissue section using GTX04468 Desmoglein 3 antibody [MSVA-543M] HistoMAX™.
Squamous cell carcinoma of the penis with intense membranous Dsg3 immunostaining of tumor cells.

Anti-Desmoglein 3 antibody [MSVA-543M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04468

GTX04468 IHC-P Image

IHC-P analysis of human esophageal squamous epithelium tissue section using GTX04468 Desmoglein 3 antibody [MSVA-543M] HistoMAX™.
In the esophagus, a strong membranous Dsg3 immunostaining predominantly occurs in the lower of the squamous epithelium.

Anti-Desmoglein 3 antibody [MSVA-543M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04468

GTX04468 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX04468 Desmoglein 3 antibody [MSVA-543M] HistoMAX™.
In the tonsil a moderate to strong Dsg3 immunostaining occurs in squamous epithelial cells of crypts. All other cell types are Dsg3 negative.

REFERENCE

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REVIEW

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Package List Price ($)
$ 629
$ 299