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E-Cadherin antibody - VetSignal

Anti-E-Cadherin antibody - VetSignal used in Western Blot (WB). GTX135001
Anti-E-Cadherin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135001
Anti-E-Cadherin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135001

Cat No. GTX135001

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Dog
Package
100 μl ($329)
See all E-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

MDCK

Predict Reactivity

Human, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog E-Cadherin.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 1 , Cadherin-1 , Uvomorulin

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody - VetSignal used in Western Blot (WB). GTX135001

GTX135001 WB Image

Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX135001) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135001

GTX135001 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX135001) diluted at 1:1000.

Anti-E-Cadherin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135001

GTX135001 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
E-Cadherin stained by E-Cadherin antibody (GTX135001) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329