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E-Cadherin antibody - VetSignal

Anti-E-Cadherin antibody - VetSignal used in Western Blot (WB). GTX135001
Anti-E-Cadherin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135001
Anti-E-Cadherin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135001

Cat No. GTX135001

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Dog
Package
100 μl ($319)
See all E-Cadherin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

MDCK

Predict Reactivity

Human, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog E-Cadherin.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cadherin 1,Cadherin-1,Uvomorulin,Cdh1

Database

Research Area

DATA IMAGES
Anti-E-Cadherin antibody - VetSignal used in Western Blot (WB). GTX135001

GTX135001 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX135001) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-E-Cadherin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135001

GTX135001 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX135001) diluted at 1:1000.
Anti-E-Cadherin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135001

GTX135001 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
E-Cadherin stained by E-Cadherin antibody (GTX135001) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319