GeneTex
United States (US)

EasyBlot Sampler Kit

Cat No. GTX300111

PRODUCT

Product Content

GTX221666-01 EasyBlot anti Rabbit IgG (HRP)
Application : WB
Reactivity : Rabbit
Package : 10 μl
GTX221667-01 EasyBlot anti Mouse IgG (HRP)
Application : WB
Reactivity : Mouse
Package : 10 μl
GTX425858 EasyBlocker
Application : WB
Package : 2 g

Product Description

EasyBlot anti-Rabbit/Mouse IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of rabbit/Mouse IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments.

Note: 1.Suggested dilution is 1:1000. Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples.
2.Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to rabbit IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.

To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Rabbit IgG (Optimized for Prot A/G) (GTXGTX225856-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Rabbit IgG Agarose beads (GTX27019) instead of Protein A/G.
PROPERTIES

Form

Liquid

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Package List Price ($)
$ 149