GeneTex
United States (US)

Fhl2a antibody

GTX124333_40135_IHC-Wm_Z_18121410_781.jpg
GTX124333_40135_20150420_IHC-Wm_Z_18121410_215.jpg
GTX124333_40135_WB_Z_18121410_138.jpg
GTX124333_40135_IHC_Z_18121410_633.jpg

Cat No. GTX124333

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Wm
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

Zebrafish heart , zebrafish embryo , Ubiquitous express
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Four And A Half Lim Domains 2A,Zgc:92428,Fhl2A

Background

Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation (By similarity). Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2.

Research Area

DATA IMAGES
GTX124333_40135_IHC-Wm_Z_18121410_781.jpg

GTX124333 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using fhl2a (GTX124333) antibody at 1:200 dilution.

GTX124333_40135_20150420_IHC-Wm_Z_18121410_215.jpg

GTX124333 IHC-Wm Image

Fhl2a antibody detects Fhl2a protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Fhl2a antibody (GTX124333) dilution: 1:100.

GTX124333_40135_WB_Z_18121410_138.jpg

GTX124333 WB Image

Sample (30 ug of whole cell lysate)
A: zebrafish heart
12% SDS PAGE
GTX124333 diluted at 1:1000

GTX124333_40135_IHC_Z_18121410_633.jpg

GTX124333 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using fhl2a (GTX124333) antibody at 1:300 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 389
$ 199