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GAD65 antibody [MSVA-602M] HistoMAX™

HistoMAX
Anti-GAD65 antibody [MSVA-602M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04477
Anti-GAD65 antibody [MSVA-602M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04477
Anti-GAD65 antibody [MSVA-602M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04477

Cat. No. GTX04477

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MSVA-602M

Isotype

 IgG2b

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($679),
100 μl ($299)
See all GAD65 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Pancreas: A strong GAD2 staining should be seen in pancreatic islet cells.

Negative Control

Colon: GAD2 immunostaining should be absent in all cell types

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate decarboxylase 2 , GAD65

Cellular Localization

Cytoplasm, cytosol , Cytoplasmic vesicle , Presynaptic cell membrane , Golgi apparatus membrane

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GAD65 antibody [MSVA-602M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04477

GTX04477 IHC-P Image

IHC-P analysis of human pancreatic neuroendocrine tumor (PNET) tissue using GTX04477 GAD65 antibody [MSVA-602M] HistoMAX™.
Pancreatic neuroendocrine tumor with strong GAD65 immunostaining of tumor cells.

Anti-GAD65 antibody [MSVA-602M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04477

GTX04477 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX04477 GAD65 antibody [MSVA-602M] HistoMAX™.
Strong cytoplasmic GAD65 staining of pancreatic islet cells.

Anti-GAD65 antibody [MSVA-602M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04477

GTX04477 IHC-P Image

IHC-P analysis of human cerebrum (grey matter) tissue using GTX04477 GAD65 antibody [MSVA-602M] HistoMAX™.
Strong GAD65 staining of nerve fibres in the grey matter of the cerebrum.

REFERENCE

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REVIEW

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Package List Price ($)
$ 679
$ 299