PRODUCT
Summary
This antibody was validated on 76 different Normal Tissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
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Not tested in other applications.
Calculated MW
Positive Control
Pancreas: A strong GAD2 staining should be seen in pancreatic islet cells.
Negative Control
Colon: GAD2 immunostaining should be absent in all cell types
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamate decarboxylase 2 , GAD65
Cellular Localization
Cytoplasm, cytosol , Cytoplasmic vesicle , Presynaptic cell membrane , Golgi apparatus membrane
Background
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
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GTX04477 IHC-P Image
IHC-P analysis of human pancreatic neuroendocrine tumor tissue section using GTX04477 GAD65 antibody [MSVA-602M] HistoMAX. Pancreatic neuroendocrine tumor with strong GAD65 immunostaining of tumor cells.
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GTX04477 IHC-P Image
IHC-P analysis of human pancreas tissue section using GTX04477 GAD65 antibody [MSVA-602M] HistoMAX. Strong cytoplasmic GAD65 staining of pancreatic islet cells.
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GTX04477 IHC-P Image
IHC-P analysis of human cerebrum tissue section using GTX04477 GAD65 antibody [MSVA-602M] HistoMAX. Strong GAD65 staining of nerve fibres in the grey matter of the cerebrum.
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REFERENCE
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REVIEW
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