PRODUCT
Summary
This antibody was validated on 76 different Normal Tissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:50-1:100 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48 and BOND RX - Leica. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Kidney: A moderate to strong nuclear staining reaction should be seen in a fraction of collecting duct cells and podocytes in glomeruli. ; Tonsil: The vast majority of T helper cells (Th2) in the T-zones should show a weak to moderate nuclear staining.
Negative Control
Kidney: Staining should be absent in proximal and distal tubuli as well as in blood vessels. ; Tonsil: Staining should be absent in B-cells.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human GATA-3 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GATA binding protein 3 , HDR , HDRS
Cellular Localization
Nucleus
Background
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
![Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420](/templates/default/js/lightbox/images/loading.gif "Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420")
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GTX04420 IHC-P Image
IHC-P analysis of human breast cancer tissue section using GTX04420 GATA3 antibody [MSVA-450M] HistoMAX.
Breast cancer of no special type NST showing moderate to strong GATA3 immunostaining.
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GTX04420 IHC-P Image
IHC-P analysis of human urinary bladder muscle invasive urothelial carcinoma tissue section using GTX04420 GATA3 antibody [MSVA-450M] HistoMAX.
Muscle invasive urothelial carcinoma with moderate to strong GATA3 immunostaining of tumor cells.
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GTX04420 IHC-P Image
IHC-P analysis of human kidney medulla tissue section using GTX04420 GATA3 antibody [MSVA-450M] HistoMAX.
In the kidney a moderate to strong GATA3 positivity is seen in collecting ducts.
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REFERENCE
There are currently no references for GATA3 antibody [MSVA-450M] HistoMAX (GTX04420). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GATA3 antibody [MSVA-450M] HistoMAX (GTX04420). Be the first to share your experience with this product.
![Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420](/upload/website/prouct_img/small/GTX04420/GTX04420_20230728_IHC-P_55_23072722_334.webp "Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420")
![Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420](/upload/website/prouct_img/small/GTX04420/GTX04420_20230728_IHC-P_185_23072723_655.webp "Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420")
![Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420](/upload/website/prouct_img/small/GTX04420/GTX04420_20230728_IHC-P_315_23072723_155.webp "Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420")
![Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420](/upload/website/prouct_img/normal/GTX04420/GTX04420_20230728_IHC-P_55_23072722_334.webp "Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420")
![Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420](/upload/website/prouct_img/normal/GTX04420/GTX04420_20230728_IHC-P_185_23072723_655.webp "Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420")
![Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420](/upload/website/prouct_img/normal/GTX04420/GTX04420_20230728_IHC-P_315_23072723_155.webp "Anti-GATA3 antibody [MSVA-450M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04420")