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Gnb1a antibody

Anti-Gnb1a antibody used in Western Blot (WB). GTX124712
Anti-Gnb1a antibody used in IHC (Paraffin sections) (IHC-P). GTX124712
Anti-Gnb1a antibody used in IHC (Whole mount) (IHC-Wm). GTX124712

Cat No. GTX124712

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr, IHC-Wm

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

zebrafish brain , zebrafish eye , whole zebrafish

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.05 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

guanine nucleotide binding protein (G protein), beta polypeptide 1a , gnb1 , wu:fb50b09 , wu:fj94h04

Background

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Database

Research Area

DATA IMAGES

Anti-Gnb1a antibody used in Western Blot (WB). GTX124712

GTX124712 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Gnb1a antibody (GTX124712) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gnb1a antibody used in IHC (Paraffin sections) (IHC-P). GTX124712

GTX124712 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using gnb1a (GTX124712) antibody at 1:300 dilution.

Anti-Gnb1a antibody used in IHC (Whole mount) (IHC-Wm). GTX124712

GTX124712 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using gnb1a (GTX124712) antibody at 1:200 dilution.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219