PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Liver: An at least moderate staining should be seen of sinusoids and Kupffer cells. Kidney: An at least moderate staining should be seen in small capillaries of glomeruli.
Negative Control
Absence of HLA-DRa staining is expected in hepatocytes of a completely normal liver and in tubuli of completely normal kidney.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human HLA-DR protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
major histocompatibility complex, class II, DR alpha , HLA-DRA1
Cellular Localization
Cell membrane; Single-pass type I membrane protein
Background
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429](/templates/default/js/lightbox/images/loading.gif "Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429")
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GTX04429 IHC-P Image
IHC-P analysis of human squamous cell carcinoma (SCC) from vulva tissue using GTX04429 HLA-DRA antibody [MSVA-470R] HistoMAX™.
Vulva Squamous cell carcinoma showing strong HLA-DRA immunostaining in tumor cells.
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GTX04429 IHC-P Image
IHC-P analysis of human liver tissue using GTX04429 HLA-DRA antibody [MSVA-470R] HistoMAX™.
Sinusoids and Kupffer cells show strong HLA-DRA immunostaining in the liver.
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GTX04429 IHC-P Image
IHC-P analysis of human colorectal adenocarcinoma (COAD, HLA-AR negative) tissue using GTX04429 HLA-DRA antibody [MSVA-470R] HistoMAX™.
HLA-DRA negative colorectal adenocarcinoma showing strong HLA-DRA positivity of capillaries and tumor associated inflammatory cells.
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REFERENCE
There are currently no references for HLA-DRA antibody [MSVA-470R] HistoMAX™ (GTX04429). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HLA-DRA antibody [MSVA-470R] HistoMAX™ (GTX04429). Be the first to share your experience with this product.
![Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429](/upload/website/prouct_img/small/GTX04429/GTX04429_20230728_IHC-P_192_23072723_449.webp "Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429")
![Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429](/upload/website/prouct_img/small/GTX04429/GTX04429_20230728_IHC-P_322_23072723_391.webp "Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429")
![Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429](/upload/website/prouct_img/small/GTX04429/GTX04429_20230728_IHC-P_62_23072722_443.webp "Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429")
![Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429](/upload/website/prouct_img/normal/GTX04429/GTX04429_20230728_IHC-P_192_23072723_449.webp "Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429")
![Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429](/upload/website/prouct_img/normal/GTX04429/GTX04429_20230728_IHC-P_322_23072723_391.webp "Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429")
![Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429](/upload/website/prouct_img/normal/GTX04429/GTX04429_20230728_IHC-P_62_23072722_443.webp "Anti-HLA-DRA antibody [MSVA-470R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04429")