GeneTex
  • Country / Location Selection

United States (US)

Hmgb1 antibody

Anti-Hmgb1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124486
Anti-Hmgb1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124486
Anti-Hmgb1 antibody used in Western Blot (WB). GTX124486
Anti-Hmgb1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124486

Cat No. GTX124486

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Wm

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

zebrafish liver

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

high mobility group box 1a , hmgb1 , ik:tdsubc_1a5 , tdsubc_1a5 , wu:fb23c02 , xx:tdsubc_1a5 , zgc:56110 , zgc:77104

Database

Research Area

DATA IMAGES

Anti-Hmgb1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124486

GTX124486 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using hmgb1 (GTX124486) antibody at 1:300 dilution.

Anti-Hmgb1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124486

GTX124486 IHC-Wm Image

Hmgb1 antibody detects Hmgb1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Hmgb1 stained by Hmgb1 antibody (GTX124486) diluted at 1:100.

Anti-Hmgb1 antibody used in Western Blot (WB). GTX124486

GTX124486 WB Image

Zebrafish tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Hmgb1 antibody (GTX124486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Hmgb1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124486

GTX124486 IHC-Wm Image

Hmgb1 antibody detects Hmgb1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Hmgb1 stained by Hmgb1 antibody (GTX124486) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

There are currently no references for Hmgb1 antibody (GTX124486). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Hmgb1 antibody (GTX124486). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219