GeneTex
United States (US)

Hmgb1 antibody

GTX124486_39742_IHC_Z_18121410_624.jpg
GTX124486_43194_20180525_WB_Z_18121410_279.jpg
GTX124486_43243_20180703_IHC-WM_Z_18121410_296.jpg
GTX124486_43362_20181115_IHC-WM_Z_19011519_563.jpg

Cat No. GTX124486

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Wm
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

zebrafish liver
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

High Mobility Group Box 1A,Hmgb1,Ik:Tdsubc_1A5,Tdsubc_1A5,Wu:Fb23C02,Xx:Tdsubc_1A5,Zgc:56110,Zgc:77104,Hmgb1A

Research Area

DATA IMAGES
GTX124486_39742_IHC_Z_18121410_624.jpg

GTX124486 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using hmgb1 (GTX124486) antibody at 1:300 dilution.

GTX124486_43194_20180525_WB_Z_18121410_279.jpg

GTX124486 WB Image

Various tissue extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hmgb1 antibody (GTX124486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124486_43243_20180703_IHC-WM_Z_18121410_296.jpg

GTX124486 IHC-Wm Image

Hmgb1 antibody detects Hmgb1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Hmgb1 stained by Hmgb1 antibody (GTX124486) diluted at 1:100.

GTX124486_43362_20181115_IHC-WM_Z_19011519_563.jpg

GTX124486 IHC-Wm Image

Hmgb1 antibody detects Hmgb1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Hmgb1 stained by Hmgb1 antibody (GTX124486) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70°C

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199