PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:75-1:150 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48 and BOND RX - Leica. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells.
Negative Control
Colon adenocarcinoma with previously documented loss of MSH6 expression: Nuclear staining should be absent in cancer cells, while a nuclear staining must be seen in stromal and inflammatory cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 1-100) of human MSH6 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mutS homolog 6 , GTBP , GTMBP , HNPCC5 , HSAP , p160
Cellular Localization
Nucleus
Background
This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
![Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416](/templates/default/js/lightbox/images/loading.gif "Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416")
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GTX04416 IHC-P Image
IHC-P analysis of human mucosa from descending colon tissue using GTX04416 MSH6 antibody [MSVA-906R] HistoMAX™.
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GTX04416 IHC-P Image
IHC-P analysis of human colorectal adenocarcinoma (COAD) tissue using GTX04416 MSH6 antibody [MSVA-906R] HistoMAX™.
Colorectal adenocarcinoma with strong MSH6 immunostaining in stromal and inflammatory cells but absent MSH6 staining in cancer cells.
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GTX04416 IHC-P Image
IHC-P analysis of human colorectal adenocarcinoma (COAD) tissue using GTX04416 MSH6 antibody [MSVA-906R] HistoMAX™.
Colorectal adenocarcinoma showing strong MSH6 positivity in cancer cells and moderate staining in stromal cells.
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REFERENCE
There are currently no references for MSH6 antibody [MSVA-906R] HistoMAX™ (GTX04416). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MSH6 antibody [MSVA-906R] HistoMAX™ (GTX04416). Be the first to share your experience with this product.
![Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416](/upload/website/prouct_img/small/GTX04416/GTX04416_20230728_IHC-P_213_23072722_619.webp "Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416")
![Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416](/upload/website/prouct_img/small/GTX04416/GTX04416_20230728_IHC-P_343_23072723_133.webp "Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416")
![Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416](/upload/website/prouct_img/small/GTX04416/GTX04416_20230728_IHC-P_83_23072722_284.webp "Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416")
![Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416](/upload/website/prouct_img/normal/GTX04416/GTX04416_20230728_IHC-P_213_23072722_619.webp "Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416")
![Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416](/upload/website/prouct_img/normal/GTX04416/GTX04416_20230728_IHC-P_343_23072723_133.webp "Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416")
![Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416](/upload/website/prouct_img/normal/GTX04416/GTX04416_20230728_IHC-P_83_23072722_284.webp "Anti-MSH6 antibody [MSVA-906R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04416")