Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 is optimal but pH 7.8 results in only slightly weaker stainings. At pH 6, the immunostaining is markedly reduced.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A fraction of squamous epithelial cells of the tonsil crypts should show strong mesothelin immunostaining. Fallopian tube: An at least moderate mesothelin positivity should be seen at the apical membrane of epithelial cells.
Negative Control
Tonsil: Squamous epithelial cells of the tonsil surface and all lymphocytes must not show mesothelin immunostaining.
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
mesothelin , MPF , SMRP
Cellular Localization
Golgi apparatus , Secreted
Background
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
Database
Research Area
![Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373](/upload/website/prouct_img/small/GTX04373/GTX04373_20230728_IHC-P_77_23072722_210.webp "Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373")
![Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373](/upload/website/prouct_img/small/GTX04373/GTX04373_20230728_IHC-P_207_23072722_613.webp "Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373")
![Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373](/upload/website/prouct_img/small/GTX04373/GTX04373_20230728_IHC-P_337_23072723_182.webp "Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373")
![Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373](/upload/website/prouct_img/normal/GTX04373/GTX04373_20230728_IHC-P_77_23072722_210.webp "Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373")
![Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373](/upload/website/prouct_img/normal/GTX04373/GTX04373_20230728_IHC-P_207_23072722_613.webp "Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373")
![Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373](/upload/website/prouct_img/normal/GTX04373/GTX04373_20230728_IHC-P_337_23072723_182.webp "Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373")