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Mesothelin antibody [MSVA-235M] HistoMAX™

HistoMAX
Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373
Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373
Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373

Cat. No. GTX04373

Host

Mouse

Clonality

Monoclonal

Clone Name

MSVA-235M

Isotype

IgG2b

Application

IHC-P

Reactivity

Human
Package
500 μl ($629),
100 μl ($299)
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 is optimal but pH 7.8 results in only slightly weaker stainings. At pH 6, the immunostaining is markedly reduced.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.

Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Tonsil: A fraction of squamous epithelial cells of the tonsil crypts should show strong mesothelin immunostaining. Fallopian tube: An at least moderate mesothelin positivity should be seen at the apical membrane of epithelial cells.

Negative Control

Tonsil: Squamous epithelial cells of the tonsil surface and all lymphocytes must not show mesothelin immunostaining.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mesothelin , MPF , SMRP

Cellular Localization

Golgi apparatus , Secreted

Background

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373

GTX04373 IHC-P Image

IHC-P analysis of human breast adenoid cystic carcinoma (ACC) tissue using GTX04373 Mesothelin antibody [MSVA-235M] HistoMAX™.
Adenoid cystic breast cancer with strong membranous mesothelin immunostaining in a subset of cells.

Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373

GTX04373 IHC-P Image

IHC-P analysis of human endometrioid ovarian cancer (EOVC) tissue using GTX04373 Mesothelin antibody [MSVA-235M] HistoMAX™.
Strong predominantly membranous mesothelin staining in an endometroid ovarian cancer.

Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373

GTX04373 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX04373 Mesothelin antibody [MSVA-235M] HistoMAX™.
Strong predominantly cytoplasmic mesothelin immunostaining in a stomach cancer with diffuse growth pattern.

REFERENCE

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REVIEW

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Package List Price ($)
$ 629
$ 299