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Mucin 5AC antibody [MSVA-109M] HistoMAX™

HistoMAX
Anti-Mucin 5AC antibody [MSVA-109M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04364
Anti-Mucin 5AC antibody [MSVA-109M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04364
Anti-Mucin 5AC antibody [MSVA-109M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04364

Cat. No. GTX04364

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MSVA-109M

Isotype

 IgG1

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($519),
100 μl ($299)
See all Mucin 5AC products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

586 kDa. ( Note )

Positive Control

Gastric mucosa: A strong cytoplasmic MUC5AC staining should be seen in the surface epithelium.

Negative Control

Kidney: MUC5AC staining should be absent.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mucin 5AC, oligomeric mucus/gel-forming , MUC5 , TBM , leB , mucin

Cellular Localization

Secreted

Background

Predicted to be an extracellular matrix structural constituent. Involved in phosphatidylinositol-mediated signaling. Located in cytoplasm; extracellular space; and mucus layer. Implicated in dry eye syndrome. Biomarker of several diseases, including Sjogren's syndrome; biliary tract disease (multiple); cystic fibrosis; eye disease (multiple); and pancreatic cancer (multiple). [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-Mucin 5AC antibody [MSVA-109M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04364

GTX04364 IHC-P Image

IHC-P analysis of human gastric antrum mucosa tissue using GTX04364 Mucin 5AC antibody [MSVA-109M] HistoMAX™.
Strong mucin 5AC staining of surface epithelial cells.

Anti-Mucin 5AC antibody [MSVA-109M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04364

GTX04364 IHC-P Image

IHC-P analysis of human duodenum tissue using GTX04364 Mucin 5AC antibody [MSVA-109M] HistoMAX™.
Mucin 5AC negative Brunner gland.

Anti-Mucin 5AC antibody [MSVA-109M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04364

GTX04364 IHC-P Image

IHC-P analysis of human gastric adenocarcinoma (100% of tumor cells) tissue using GTX04364 Mucin 5AC antibody [MSVA-109M] HistoMAX™.
Strong MUC5AC immunostaining in an adenocarcinoma of the stomach 100% of tumor cells.

REFERENCE

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REVIEW

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Package List Price ($)
$ 519
$ 299