PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
|
Not tested in other applications.
Calculated MW
Positive Control
Lung: Nuclear TTF1 staining should be strong in pneumocytes and in basal cells of the terminal bronchioles while a weaker, still moderate to strong staining should be seen in columnar epithelial cells of the terminal bronchioles (low expressor control).
Negative Control
Liver: TTF1 staining must not be seen in any cells.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human TTF-1 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NK2 homeobox 1 , BCH , BHC , NK-2 , NKX2.1 , NKX2A , NMTC1 , T/EBP , TEBP , TITF1 , TTF-1 , TTF1
Cellular Localization
Nucleus
Background
This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Feb 2014]
Database
Research Area
DATA IMAGES
![Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489](/templates/default/js/lightbox/images/loading.gif "Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489")
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GTX04489 IHC-P Image
IHC-P analysis of human pneumocytes tissue section using GTX04489 NKX2.1 antibody [MSVA-312R] HistoMAX™.
Intense NKX2.1 positivity of pneumocytes
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GTX04489 IHC-P Image
IHC-P analysis of human pulmonary adenocarcinoma tissue section using GTX04489 NKX2.1 antibody [MSVA-312R] HistoMAX™.
Pulmonary adenocarcinoma with strong NKX2.1 staining of all tumor cells.
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GTX04489 IHC-P Image
IHC-P analysis of human thyroid gland tissue section using GTX04489 NKX2.1 antibody [MSVA-312R] HistoMAX™.
Thyroid gland with strong NKX2.1 staining of follicular epithelial cells.
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REFERENCE
There are currently no references for NKX2.1 antibody [MSVA-312R] HistoMAX™ (GTX04489). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NKX2.1 antibody [MSVA-312R] HistoMAX™ (GTX04489). Be the first to share your experience with this product.
![Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489](/upload/website/prouct_img/small/GTX04489/GTX04489_20230728_IHC-P_382_23072723_485.webp "Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489")
![Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489](/upload/website/prouct_img/small/GTX04489/GTX04489_20230728_IHC-P_122_23072722_461.webp "Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489")
![Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489](/upload/website/prouct_img/small/GTX04489/GTX04489_20230728_IHC-P_252_23072723_487.webp "Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489")
![Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489](/upload/website/prouct_img/normal/GTX04489/GTX04489_20230728_IHC-P_382_23072723_485.webp "Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489")
![Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489](/upload/website/prouct_img/normal/GTX04489/GTX04489_20230728_IHC-P_122_23072722_461.webp "Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489")
![Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489](/upload/website/prouct_img/normal/GTX04489/GTX04489_20230728_IHC-P_252_23072723_487.webp "Anti-NKX2.1 antibody [MSVA-312R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04489")