PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Kidney: an at least moderate granular cytoplasmic staining should be seen in proximal tubule cells
Negative Control
Colon: staining should be absent in epithelial and other cells of the lamina propria.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human Napsin-A protein fragment (aa189-299)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
napsin A aspartic peptidase , KAP , Kdap , NAP1 , NAPA , SNAPA
Cellular Localization
Secreted
Background
This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. This gene has been described as a marker for lung adenocarcinoma and renal cell carcinoma. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
![Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366](/templates/default/js/lightbox/images/loading.gif "Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366")
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GTX04366 IHC-P Image
IHC-P analysis of human lung tissue section using GTX04366 Napsin A antibody [MSVA-555R] HistoMAX™.
Normal lung showing strong Napsin A immunostaining of pneumocytes and alveolar macrophages.
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GTX04366 IHC-P Image
IHC-P analysis of human kidney tissue section using GTX04366 Napsin A antibody [MSVA-555R] HistoMAX™.
Moderate Napsin A immunostaining in proximal tubuli of the kidney.
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GTX04366 IHC-P Image
IHC-P analysis of human lung adenocarcinoma tissue section using GTX04366 Napsin A antibody [MSVA-555R] HistoMAX™.
Adenocarcinoma of the lung with strong expression of Napsin A.
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REFERENCE
There are currently no references for Napsin A antibody [MSVA-555R] HistoMAX™ (GTX04366). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Napsin A antibody [MSVA-555R] HistoMAX™ (GTX04366). Be the first to share your experience with this product.
![Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366](/upload/website/prouct_img/small/GTX04366/GTX04366_20230728_IHC-P_345_23072723_132.webp "Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366")
![Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366](/upload/website/prouct_img/small/GTX04366/GTX04366_20230728_IHC-P_85_23072722_477.webp "Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366")
![Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366](/upload/website/prouct_img/small/GTX04366/GTX04366_20230728_IHC-P_215_23072722_558.webp "Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366")
![Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366](/upload/website/prouct_img/normal/GTX04366/GTX04366_20230728_IHC-P_345_23072723_132.webp "Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366")
![Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366](/upload/website/prouct_img/normal/GTX04366/GTX04366_20230728_IHC-P_85_23072722_477.webp "Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366")
![Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366](/upload/website/prouct_img/normal/GTX04366/GTX04366_20230728_IHC-P_215_23072722_558.webp "Anti-Napsin A antibody [MSVA-555R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04366")