PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:75-1:150 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including BOND RX - Leica and Roche - Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Positive Control
Placenta: Trophoplast and chorion cells should show a strong cytoplasmic papp-A staining.
Negative Control
Colon: Papp-A immunostaining should be completely absent in both stromal end epithelial cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (within aa 351-523) of human PAPP-A protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
pappalysin 1 , ASBABP2 , DIPLA1 , IGFBP-4ase , PAPA , PAPP-A , PAPPA1
Cellular Localization
Secreted
Background
This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437](/templates/default/js/lightbox/images/loading.gif "Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437")
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GTX04437 IHC-P Image
IHC-P analysis of human placenta tissue using GTX04437 PAPP A antibody [MSVA-780M] HistoMAX™.
Strong papp A immunostaining of trophoblast cells of a first trimenon placenta.
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GTX04437 IHC-P Image
IHC-P analysis of human mucosa from appendix tissue using GTX04437 PAPP A antibody [MSVA-780M] HistoMAX™.
Complete absence of papp A immunostaining in epithelial and stromal cells of the appendix mucosa.
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GTX04437 IHC-P Image
IHC-P analysis of human placenta (mature) tissue using GTX04437 PAPP A antibody [MSVA-780M] HistoMAX™.
Syncytiotrophoblast cells show strong papp A immunostaining in a mature placenta.
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REFERENCE
There are currently no references for PAPP A antibody [MSVA-780M] HistoMAX™ (GTX04437). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PAPP A antibody [MSVA-780M] HistoMAX™ (GTX04437). Be the first to share your experience with this product.
![Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437](/upload/website/prouct_img/small/GTX04437/GTX04437_20230728_IHC-P_92_23072722_835.webp "Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437")
![Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437](/upload/website/prouct_img/small/GTX04437/GTX04437_20230728_IHC-P_222_23072722_333.webp "Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437")
![Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437](/upload/website/prouct_img/small/GTX04437/GTX04437_20230728_IHC-P_352_23072723_218.webp "Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437")
![Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437](/upload/website/prouct_img/normal/GTX04437/GTX04437_20230728_IHC-P_92_23072722_835.webp "Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437")
![Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437](/upload/website/prouct_img/normal/GTX04437/GTX04437_20230728_IHC-P_222_23072722_333.webp "Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437")
![Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437](/upload/website/prouct_img/normal/GTX04437/GTX04437_20230728_IHC-P_352_23072723_218.webp "Anti-PAPP A antibody [MSVA-780M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04437")