PRODUCT
Summary
This antibody was validated on 76 different Normal Tissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
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Not tested in other applications.
Calculated MW
Positive Control
Pancreas: A strong PAX6 positivity should be seen in the cells of islets of Langerhans.
Negative Control
Tonsil: PAX6 immunostaining should be absent in all cells.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human PAX6 fragment
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
paired box 6 , AN , AN2 , ASGD5 , D11S812E , FVH1 , MGDA , WAGR
Cellular Localization
Nucleus
Background
This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]
Database
Research Area
DATA IMAGES
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GTX04487 IHC-P Image
IHC-P analysis of human pancreatic neuroendocrine tumor tissue section using GTX04487 PAX6 antibody [MSVA-706M] HistoMAX. Pancreatic neuroendocrine tumor with strong predominantly nuclear Pax6 positivity of all tumor cells.
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GTX04487 IHC-P Image
IHC-P analysis of human pancreas tissue section using GTX04487 PAX6 antibody [MSVA-706M] HistoMAX. Pancreas with intense nuclear Pax6 staining of islet cells scaled.
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GTX04487 IHC-P Image
IHC-P analysis of human cerebellum tissue section using GTX04487 PAX6 antibody [MSVA-706M] HistoMAX. Cerebellum with nuclear Pax6 staining of granule cells scaled.
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REFERENCE
There are currently no references for PAX6 antibody [MSVA-706M] HistoMAX (GTX04487). Be the first to share your publications with this product.
REVIEW
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