PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Prostate: The epithelial cells of the prostate glands must show a strong cytoplasmic staining. Due to leakage of the antigen in the vicinity of the prostate glands, adjacent stroma cells may display a weak to moderate staining reaction.
Negative Control
Colon: PSA immunostaining should be absent in all cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa150-250) of human KLK3 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
kallikrein related peptidase 3 , APS , KLK2A1 , PSA , hK3
Cellular Localization
Secreted
Background
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481](/templates/default/js/lightbox/images/loading.gif "Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481")
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GTX04481 IHC-P Image
IHC-P analysis of human prostatic adenocarcinoma (PAC) tissue with (Gleason 3+3=6) tissue using GTX04481 PSA antibody [MSVA-603R] HistoMAX™.
Prostate Adenocarcinoma (Gleason 3+3=6) showing strong PSA positivity of tumor cells.
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GTX04481 IHC-P Image
IHC-P analysis of human kidney tissue using GTX04481 PSA antibody [MSVA-603R] HistoMAX™.
Absence of PSA immunostaining.
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GTX04481 IHC-P Image
IHC-P analysis of human prostatic adenocarcinoma (PAC) tissue with (Gleason 3+3=6) tissue using GTX04481 PSA antibody [MSVA-603R] HistoMAX™.
Prostate Adenocarcinoma (Gleason 3+3=6) with strong PSA immunostaining of all tumor cells.
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REFERENCE
There are currently no references for PSA antibody [MSVA-603R] HistoMAX™ (GTX04481). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PSA antibody [MSVA-603R] HistoMAX™ (GTX04481). Be the first to share your experience with this product.
![Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481](/upload/website/prouct_img/small/GTX04481/GTX04481_20230728_IHC-P_101_23072722_943.webp "Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481")
![Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481](/upload/website/prouct_img/small/GTX04481/GTX04481_20230728_IHC-P_231_23072723_230.webp "Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481")
![Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481](/upload/website/prouct_img/small/GTX04481/GTX04481_20230728_IHC-P_361_23072723_287.webp "Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481")
![Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481](/upload/website/prouct_img/normal/GTX04481/GTX04481_20230728_IHC-P_101_23072722_943.webp "Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481")
![Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481](/upload/website/prouct_img/normal/GTX04481/GTX04481_20230728_IHC-P_231_23072723_230.webp "Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481")
![Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481](/upload/website/prouct_img/normal/GTX04481/GTX04481_20230728_IHC-P_361_23072723_287.webp "Anti-PSA antibody [MSVA-603R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04481")