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Rat Carbonic Anhydrase II protein (active)

Rat Carbonic Anhydrase II protein (active). GTX00347-pro
Rat Carbonic Anhydrase II protein (active). GTX00347-pro

Cat No. GTX00347-pro

Application Functional Assay
Species Rat
Package
10 μg ($219)
APPLICATION

Application Note

CA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration of carbon dioxide. It is essential for bone resorption and osteoclast differentiation and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It is widely accepted that CA2 also catalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay was conducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA) as substrate. Briefly, different concentrations of CA2 were incubated with 1mM 4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was read per hour that CA2 catalyzes hydrolysis of p-Nitrophenyl Acetate.

Observed MW

29 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Native Protein (NP_062164.1)

Expression System

native protein from rat (Erythrocytes)

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

carbonic anhydrase 2,Ca2

Background

putative carbonic anhydrase; human homolog catalyzes the reversible hydration of carbon dioxide [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat Carbonic Anhydrase II protein (active). GTX00347-pro

GTX00347-pro Functional Assay Image

Hydrolysis of p-Nitrophenyl Acetate catalyzed by various concentrations of GTX00347-pro Rat Carbonic Anhydrase II protein (active).

Rat Carbonic Anhydrase II protein (active). GTX00347-pro

GTX00347-pro Image

SDS-PAGE analysis of GTX00347-pro Rat Carbonic Anhydrase II protein (active).

REFERENCE
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Package List Price ($)
$ 219