GeneTex
United States (US)

SHP1 antibody

GTX102865_WB_19011506_781.jpg
GTX102865_IFA_19011506_843.jpg
GTX102865_WB_M_19011506_284.jpg

Cat No. GTX102865

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , NIH-3T3 , *U2OS

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SHP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Tyrosine Phosphatase, Non-Receptor Type 6,Hcp,Hcph,Hptp1C,Ptp-1C,Sh-Ptp1,Shp-1,Shp-1L,Shp1,Ptpn6

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102865_WB_19011506_781.jpg

GTX102865 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX102865 diluted at 1:500

GTX102865_IFA_19011506_843.jpg

GTX102865 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SHP1(GTX102865) antibody at 1:200 dilution.

GTX102865_WB_M_19011506_284.jpg

GTX102865 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX102865 diluted at 1:1000

REVIEW

SHP1 antibody Cat No. GTX102865

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 07-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169