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SOX9 antibody [MSVA-709R] HistoMAX™

Recombinant antibody HistoMAX
Anti-SOX9 antibody [MSVA-709R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04459
Anti-SOX9 antibody [MSVA-709R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04459
Anti-SOX9 antibody [MSVA-709R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04459

Cat. No. GTX04459

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 MSVA-709R

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($519),
100 μl ($299)
See all SOX9 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:50-1:75

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base.

Negative Control

Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres – if present).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant humanSOX9 protein fragment (around aa 393- 508)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 9 , CMD1 , CMPD1 , SRA1 , SRXX2 , SRXY10

Cellular Localization

Nucleus

Background

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOX9 antibody [MSVA-709R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04459

GTX04459 IHC-P Image

IHC-P analysis of human esophageal squamous cell carcinoma (ESCC) tissue using GTX04459 SOX9 antibody [MSVA-709R] HistoMAX™.
Squamous cell carcinoma with strong SOX9 immunostaining of most tumor cells.

Anti-SOX9 antibody [MSVA-709R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04459

GTX04459 IHC-P Image

IHC-P analysis of human mucosa from duodenum tissue using GTX04459 SOX9 antibody [MSVA-709R] HistoMAX™.
Strong nuclear SOX9 staining of crypt epithelial cells. The SOX9 staining intensity markedly decreases towards the surface epithelium and the tips of the villosities remain SOX9 negative.

Anti-SOX9 antibody [MSVA-709R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04459

GTX04459 IHC-P Image

IHC-P analysis of human testis tissue using GTX04459 SOX9 antibody [MSVA-709R] HistoMAX™.
Strong SOX9 staining of Sertoli cells.

REFERENCE

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REVIEW

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Package List Price ($)
$ 519
$ 299