PRODUCT
Summary
This antibody was validated on 76 different Normal Tissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:50-1:75 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
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Not tested in other applications.
Calculated MW
Positive Control
Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base.
Negative Control
Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres – if present).
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant humanSOX9 protein fragment (around aa 393- 508)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SRY-box 9 , CMD1 , CMPD1 , SRA1 , SRXX2 , SRXY10
Cellular Localization
Nucleus
Background
The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04459 IHC-P Image
IHC-P analysis of human testis tissue section using GTX04459 SOX9 antibody [MSVA-709R] HistoMAX. Strong SOX9 staining of Sertoli cells.
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GTX04459 IHC-P Image
IHC-P analysis of human esophageal squamous cell carcinoma tissue section using GTX04459 SOX9 antibody [MSVA-709R] HistoMAX. Squamous cell carcinoma with strong SOX9 immunostaining of most tumor cells.
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GTX04459 IHC-P Image
IHC-P analysis of human duodenum mucosa tissue section using GTX04459 SOX9 antibody [MSVA-709R] HistoMAX.
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REFERENCE
There are currently no references for SOX9 antibody [MSVA-709R] HistoMAX (GTX04459). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SOX9 antibody [MSVA-709R] HistoMAX (GTX04459). Be the first to share your experience with this product.