PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A moderate to strong TIM-3 immunostaining should be seen in a significant fraction of T-cells, while only few TIM-3 positive cells are seen in the germinal centre and the mantle zone.
Negative Control
Tonsil: Epithelial cells should be negative.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human TIM3 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hepatitis A virus cellular receptor 2 , CD366 , HAVcr-2 , KIM-3 , TIM3 , TIMD-3 , TIMD3 , Tim-3
Cellular Localization
Membrane , Cell junction
Background
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
![Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440](/templates/default/js/lightbox/images/loading.gif "Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440")
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GTX04440 IHC-P Image
IHC-P analysis of human Alveolar macrophages (AM) using GTX04440 TIM-3 antibody [MSVA-366R] HistoMAX™.
Alveolar macrophages show strong TIM-3 immunostaining.
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GTX04440 IHC-P Image
IHC-P analysis of human mucosa from descending colon tissue using GTX04440 TIM-3 antibody [MSVA-366R] HistoMAX™.
Colon descendens mucosa.
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GTX04440 IHC-P Image
IHC-P analysis of human squamous cell carcinoma (SCC) from uterine tissue using GTX04440 TIM-3 antibody [MSVA-366R] HistoMAX™.
TIM-3 negative squamous cell carcinoma showing a large number of TIM-3 positive macrophages and also lymphocytes.
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REFERENCE
There are currently no references for TIM-3 antibody [MSVA-366R] HistoMAX™ (GTX04440). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TIM-3 antibody [MSVA-366R] HistoMAX™ (GTX04440). Be the first to share your experience with this product.
![Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440](/upload/website/prouct_img/small/GTX04440/GTX04440_20230728_IHC-P_248_23072722_682.webp "Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440")
![Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440](/upload/website/prouct_img/small/GTX04440/GTX04440_20230728_IHC-P_378_23072723_657.webp "Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440")
![Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440](/upload/website/prouct_img/small/GTX04440/GTX04440_20230728_IHC-P_118_23072722_101.webp "Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440")
![Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440](/upload/website/prouct_img/normal/GTX04440/GTX04440_20230728_IHC-P_248_23072722_682.webp "Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440")
![Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440](/upload/website/prouct_img/normal/GTX04440/GTX04440_20230728_IHC-P_378_23072723_657.webp "Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440")
![Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440](/upload/website/prouct_img/normal/GTX04440/GTX04440_20230728_IHC-P_118_23072722_101.webp "Anti-TIM-3 antibody [MSVA-366R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04440")