PRODUCT
Summary
This antibody was validated on 76 different Normal and cancerTissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
Urinary bladder: A strong membranous and cytoplasmic Upk1a immunostaining should be seen in the urothelium (the staining can be limited to the top 1/3 or 2/3 of the urothelium).
Negative Control
Colon: Upk1a immunostaining should be absent in all cells of the colon mucosa.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
uroplakin 1A , TSPAN21 , UP1A , UPIA , UPKA
Cellular Localization
Membrane; Multi-pass membrane protein
Background
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
![Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462](/templates/default/js/lightbox/images/loading.gif "Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462")
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GTX04462 IHC-P Image
IHC-P analysis of human urinary bladder urothelium tissue section using GTX04462 UPK1A antibody [MSVA-735M] HistoMAX™.
Strong UPK1A immunostaining of the urothelium.
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GTX04462 IHC-P Image
IHC-P analysis of human kidney Invasive urothelial carcinoma tissue section using GTX04462 UPK1A antibody [MSVA-735M] HistoMAX™.
Invasive urothelial carcinoma of the kidney pelvis with strong UPK1A positivity.
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GTX04462 IHC-P Image
IHC-P analysis of human rectum mucosa tissue section using GTX04462 UPK1A antibody [MSVA-735M] HistoMAX™.
Complete absence of UPK1A immunostaining in rectal mucosa.
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REFERENCE
There are currently no references for UPK1A antibody [MSVA-735M] HistoMAX™ (GTX04462). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for UPK1A antibody [MSVA-735M] HistoMAX™ (GTX04462). Be the first to share your experience with this product.
![Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462](/upload/website/prouct_img/small/GTX04462/GTX04462_20230728_IHC-P_123_23072722_619.webp "Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462")
![Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462](/upload/website/prouct_img/small/GTX04462/GTX04462_20230728_IHC-P_253_23072723_763.webp "Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462")
![Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462](/upload/website/prouct_img/small/GTX04462/GTX04462_20230728_IHC-P_383_23072723_597.webp "Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462")
![Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462](/upload/website/prouct_img/normal/GTX04462/GTX04462_20230728_IHC-P_123_23072722_619.webp "Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462")
![Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462](/upload/website/prouct_img/normal/GTX04462/GTX04462_20230728_IHC-P_253_23072723_763.webp "Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462")
![Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462](/upload/website/prouct_img/normal/GTX04462/GTX04462_20230728_IHC-P_383_23072723_597.webp "Anti-UPK1A antibody [MSVA-735M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04462")