PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Positive Control
Urinary bladder: A moderate to strong membranous Upk3b immunostaining should be seen in umbrella cells of the normal urothelium (the staining is usually limited to apical surface membrane of umbrella cells).
Negative Control
Colon: Upk3b immunostaining should be absent in all cells of the colon mucosa.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human UPK3B fragment
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
uroplakin 3B , P35 , UP3B , UPIIIB
Cellular Localization
Cell membrane
Background
UPK3B is a minor component of the apical plaques of mammalian urothelium that binds and dimerizes with uroplakin-1b (UPK1B; MIM 602380), one of the major conserved urothelium membrane proteins. The other major conserved integral membrane proteins of urothelial plaques are UPK1A (MIM 611557), UPK2 (MIM 611558), and UPK3A (MIM 611559) (Deng et al., 2002 [PubMed 12446744]).[supplied by OMIM, Mar 2008]
Database
DATA IMAGES
![Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476](/templates/default/js/lightbox/images/loading.gif "Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476")
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GTX04476 IHC-P Image
IHC-P analysis of human epithelioid mesothelioma tissue using GTX04476 UPK3B antibody [MSVA-736M] HistoMAX™.
Epitheloid mesothelioma with membranous UPK3B staining of variable intensity in most tumor cells.
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GTX04476 IHC-P Image
IHC-P analysis of human peritoneal mesothelium tissue using GTX04476 UPK3B antibody [MSVA-736M] HistoMAX™.
Distinct Upk3b immunostaining of apical membranes of peritoneal mesothelial cells.
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GTX04476 IHC-P Image
IHC-P analysis of human urinary bladder tissue using GTX04476 UPK3B antibody [MSVA-736M] HistoMAX™.
Urothelium with distinct UPK3B immunostaining of the apical membranes of umbrella cells.
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REFERENCE
There are currently no references for UPK3B antibody [MSVA-736M] HistoMAX™ (GTX04476). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for UPK3B antibody [MSVA-736M] HistoMAX™ (GTX04476). Be the first to share your experience with this product.
![Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476](/upload/website/prouct_img/small/GTX04476/GTX04476_20230728_IHC-P_125_23072722_340.webp "Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476")
![Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476](/upload/website/prouct_img/small/GTX04476/GTX04476_20230728_IHC-P_255_23072723_350.webp "Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476")
![Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476](/upload/website/prouct_img/small/GTX04476/GTX04476_20230728_IHC-P_385_23072723_823.webp "Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476")
![Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476](/upload/website/prouct_img/normal/GTX04476/GTX04476_20230728_IHC-P_125_23072722_340.webp "Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476")
![Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476](/upload/website/prouct_img/normal/GTX04476/GTX04476_20230728_IHC-P_255_23072723_350.webp "Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476")
![Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476](/upload/website/prouct_img/normal/GTX04476/GTX04476_20230728_IHC-P_385_23072723_823.webp "Anti-UPK3B antibody [MSVA-736M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04476")