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Vimentin antibody - VetSignal

Anti-Vimentin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135083
Anti-Vimentin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135083
Anti-Vimentin antibody - VetSignal used in Western Blot (WB). GTX135083

Cat No. GTX135083

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Cat, Dog
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Bovine, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog Vimentin.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vimentin

Database

Research Area

DATA IMAGES
Anti-Vimentin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135083

GTX135083 IHC-P Image

Vimentin antibody detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog pancreas.
Vimentin stained by Vimentin antibody (GTX135083) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vimentin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135083

GTX135083 ICC/IF Image

Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody (GTX135083) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Vimentin antibody - VetSignal used in Western Blot (WB). GTX135083

GTX135083 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX135083) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319