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ZAP70 antibody [MSVA-870R] HistoMAX™

Recombinant antibody HistoMAX
Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441
Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441
Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441

Cat. No. GTX04441

Host

Rabbit

Clonality

Monoclonal

Clone Name

MSVA-870R

Isotype

IgG

Application

IHC-P

Reactivity

Human
Package
500 μl ($679),
100 μl ($299)
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:75-1:150

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Tonsil: A large fraction of the lymphocytes in the interfollicular areas and few lymphocytes in the germinal centres should show a strong ZAP70 immunostaining.

Negative Control

Tonsil: ZAP70 immunostaining should be absent in epithelial tissues and the majority of cells in germinal centres.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 247-382) ofhuman ZAP70 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zeta chain of T cell receptor associated protein kinase 70 , ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP-70

Cellular Localization

Cytoplasm , Cell membrane

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441

GTX04441 IHC-P Image

IHC-P analysis of human lymph node tissue section using GTX04441 ZAP70 antibody [MSVA-870R] HistoMAX™.
In lymph nodes ZAP70 positive lymphocytes predominate in the interfollicular zone but are much less numerous in germinal centres.

Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441

GTX04441 IHC-P Image

IHC-P analysis of human B-cell chronic lymphocytic leukemia tissue section using GTX04441 ZAP70 antibody [MSVA-870R] HistoMAX™.
B-CLL with strong ZAP70 staining of tumor cells and even stronger staining in interspersed normal T lymphocytes.

Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441

GTX04441 IHC-P Image

IHC-P analysis of human B-cell chronic lymphocytic leukemia tissue section using GTX04441 ZAP70 antibody [MSVA-870R] HistoMAX™.
ZAP70 negative B-CLL containing a significant number of ZAP70 positive T lymphocytes.

REFERENCE

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REVIEW

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Package List Price ($)
$ 679
$ 299