PRODUCT
Summary
This antibody was validated on 76 different Normal and cancerTissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:75-1:150 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A large fraction of the lymphocytes in the interfollicular areas and few lymphocytes in the germinal centres should show a strong ZAP70 immunostaining.
Negative Control
Tonsil: ZAP70 immunostaining should be absent in epithelial tissues and the majority of cells in germinal centres.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 247-382) ofhuman ZAP70 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
zeta chain of T cell receptor associated protein kinase 70 , ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP-70
Cellular Localization
Cytoplasm , Cell membrane
Background
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441](/templates/default/js/lightbox/images/loading.gif "Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441")
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GTX04441 IHC-P Image
IHC-P analysis of human B-cell chronic lymphocytic leukemia tissue section using GTX04441 ZAP70 antibody [MSVA-870R] HistoMAX™.
ZAP70 negative B-CLL containing a significant number of ZAP70 positive T lymphocytes.
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GTX04441 IHC-P Image
IHC-P analysis of human lymph node tissue section using GTX04441 ZAP70 antibody [MSVA-870R] HistoMAX™.
In lymph nodes ZAP70 positive lymphocytes predominate in the interfollicular zone but are much less numerous in germinal centres.
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GTX04441 IHC-P Image
IHC-P analysis of human B-cell chronic lymphocytic leukemia tissue section using GTX04441 ZAP70 antibody [MSVA-870R] HistoMAX™.
B-CLL with strong ZAP70 staining of tumor cells and even stronger staining in interspersed normal T lymphocytes.
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REFERENCE
There are currently no references for ZAP70 antibody [MSVA-870R] HistoMAX™ (GTX04441). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ZAP70 antibody [MSVA-870R] HistoMAX™ (GTX04441). Be the first to share your experience with this product.
![Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441](/upload/website/prouct_img/small/GTX04441/GTX04441_20230728_IHC-P_390_23072723_539.webp "Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441")
![Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441](/upload/website/prouct_img/small/GTX04441/GTX04441_20230728_IHC-P_130_23072722_719.webp "Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441")
![Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441](/upload/website/prouct_img/small/GTX04441/GTX04441_20230728_IHC-P_260_23072723_800.webp "Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441")
![Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441](/upload/website/prouct_img/normal/GTX04441/GTX04441_20230728_IHC-P_390_23072723_539.webp "Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441")
![Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441](/upload/website/prouct_img/normal/GTX04441/GTX04441_20230728_IHC-P_130_23072722_719.webp "Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441")
![Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441](/upload/website/prouct_img/normal/GTX04441/GTX04441_20230728_IHC-P_260_23072723_800.webp "Anti-ZAP70 antibody [MSVA-870R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04441")