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ZO-1 antibody - VetSignal

Anti-ZO-1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135089
Anti-ZO-1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135089
Anti-ZO-1 antibody - VetSignal used in Western Blot (WB). GTX135089

Cat No. GTX135089

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Dog
Package
100 μl ($329)
See all ZO-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

198 kDa. ( Note )

Predict Reactivity

Human, Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to dog ZO-1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tight junction protein 1 , ZO-1 , ZO1

Cellular Localization

Cell membrane,Cell junction

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135089

GTX135089 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX135089) diluted at 1:1000.

Anti-ZO-1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135089

GTX135089 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX135089) diluted at 1:1000.

Anti-ZO-1 antibody - VetSignal used in Western Blot (WB). GTX135089

GTX135089 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX135089) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329