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ZO-1 antibody - VetSignal

Anti-ZO-1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135089
Anti-ZO-1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135089
Anti-ZO-1 antibody - VetSignal used in Western Blot (WB). GTX135089

Cat No. GTX135089

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Dog
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

198 kDa. ( Note )

Predict Reactivity

Human, Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to dog ZO-1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887441

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tight junction protein 1 , ZO-1 , ZO1

Cellular Localization

Cell membrane,Cell junction

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135089

GTX135089 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX135089) diluted at 1:1000.

Anti-ZO-1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135089

GTX135089 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX135089) diluted at 1:1000.

Anti-ZO-1 antibody - VetSignal used in Western Blot (WB). GTX135089

GTX135089 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX135089) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329