APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:5000-1:20000 |
Assay dependent |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
MDCK
Predict Reactivity
Human, Mouse, Rat, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Synthetic peptide corresponding to dog beta Actin.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888492
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
actin beta
Cellular Localization
Cytoplasm, cytoskeleton,Nucleus
Database
Research Area
DATA IMAGES
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GTX634934 IHC-P Image
beta Actin antibody [GT5512] detects beta Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog muscle.beta Actin stained by beta Actin antibody [GT5512] (GTX634934) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634934 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX634934) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634934 ICC/IF Image
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.Sample: PG-4 cells were fixed in ice-cold MeOH for 5 min.Green: beta Actin stained by beta Actin antibody (GTX634934) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX634934 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX634934) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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