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beta Actin antibody [GT5512] - VetSignal

Anti-beta Actin antibody [GT5512] - VetSignal used in Western Blot (WB). GTX634934
Anti-beta Actin antibody [GT5512] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634934
Anti-beta Actin antibody [GT5512] - VetSignal used in Western Blot (WB). GTX634934
Anti-beta Actin antibody [GT5512] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX634934

Cat No. GTX634934

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT5512

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Cat, Dog
Package
100 μl ($329)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptide corresponding to dog beta Actin.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888492

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin beta

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody [GT5512] - VetSignal used in Western Blot (WB). GTX634934

GTX634934 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX634934) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634934

GTX634934 ICC/IF Image

beta Actin antibody  detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: PG-4 cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody  (GTX634934) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-beta Actin antibody [GT5512] - VetSignal used in Western Blot (WB). GTX634934

GTX634934 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX634934) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX634934

GTX634934 IHC-P Image

beta Actin antibody [GT5512] detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
beta Actin stained by beta Actin antibody [GT5512] (GTX634934) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329