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mCherry antibody

Anti-mCherry antibody used in Western Blot (WB). GTX59788

Cat No. GTX59788

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF
Package
200 μl ($319)

APPLICATION

Application Note

Recommended Starting Dilutions:
WB: Use at 0.5-4 μg/ml
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Positive Control

Recombinant mCherry can be used as a positive control.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mCherry red fluorescent protein , red fluorescent protein mCherry

Background

mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. The recombinant mCherry is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence intensity. mCherry protein is a 28.8 kDa monomer with 256 amino acid.

Research Area

DATA IMAGES

Anti-mCherry antibody used in Western Blot (WB). GTX59788

GTX59788 WB Image

Western blot analysis using recombinant mCherry. Lane 1: 10 ng Lane 2: 20 ng Lane 3: 50 ng Lane 4: 100 ng

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319