PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: More than 20 % of germinal centre B-cells must show a weak to moderate nuclear staining.
Negative Control
Colon: Luminal epithelial cells must remain p53 negative.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human full-length TP53 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Cellular Localization
Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
![Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482](/templates/default/js/lightbox/images/loading.gif "Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482")
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GTX04482 IHC-P Image
IHC-P analysis of human urinary bladder tissue section using GTX04482 p53 antibody [MSVA-053R] HistoMAX™.
Strong nuclear p53 immunostaining of all tumor cells of a muscle invasive urothelial carcinoma p53 immunohistochemistry.
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GTX04482 IHC-P Image
IHC-P analysis of human skin tissue section using GTX04482 p53 antibody [MSVA-053R] HistoMAX™.
Weak nuclear p53 immunostaining of a fraction of basal and suprabasal cells p53 immunohistochemistry scaled.
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GTX04482 IHC-P Image
IHC-P analysis of human ovary serous high grade carcinoma tissue section using GTX04482 p53 antibody [MSVA-053R] HistoMAX™.
Serous high grade carcinoma with strong nuclear p53 positivity of all tumor cells p53 immunohistochemistry.
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REFERENCE
There are currently no references for p53 antibody [MSVA-053R] HistoMAX™ (GTX04482). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p53 antibody [MSVA-053R] HistoMAX™ (GTX04482). Be the first to share your experience with this product.
![Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482](/upload/website/prouct_img/small/GTX04482/GTX04482_20230728_IHC-P_90_23072722_819.webp "Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482")
![Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482](/upload/website/prouct_img/small/GTX04482/GTX04482_20230728_IHC-P_220_23072723_353.webp "Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482")
![Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482](/upload/website/prouct_img/small/GTX04482/GTX04482_20230728_IHC-P_350_23072723_603.webp "Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482")
![Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482](/upload/website/prouct_img/normal/GTX04482/GTX04482_20230728_IHC-P_90_23072722_819.webp "Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482")
![Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482](/upload/website/prouct_img/normal/GTX04482/GTX04482_20230728_IHC-P_220_23072723_353.webp "Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482")
![Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482](/upload/website/prouct_img/normal/GTX04482/GTX04482_20230728_IHC-P_350_23072723_603.webp "Anti-p53 antibody [MSVA-053R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04482")