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Smoothened homolog antibody

Anti-Smoothened homolog antibody used in IHC (Paraffin sections) (IHC-P). GTX02530
Anti-Smoothened homolog antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02530
Anti-Smoothened homolog antibody used in Western Blot (WB). GTX02530
Anti-Smoothened homolog antibody used in IHC (Paraffin sections) (IHC-P). GTX02530
Anti-Smoothened homolog antibody used in IHC (Paraffin sections) (IHC-P). GTX02530
Anti-Smoothened homolog antibody used in IHC (Paraffin sections) (IHC-P). GTX02530

Cat. No. GTX02530

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Smoothened homolog products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human SMO, corresponding to a region within N-terminal amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

smoothened, frizzled class receptor , CRJS , FZD11 , Gx , SMOH

Cellular Localization

Membrane

Background

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Smoothened homolog antibody used in IHC (Paraffin sections) (IHC-P). GTX02530

GTX02530 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX02530 Smoothened homolog antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Smoothened homolog antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02530

GTX02530 ICC/IF Image

ICC/IF analysis of PFA-fixed HUVEC cells using GTX02530 Smoothened homolog antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-Smoothened homolog antibody used in Western Blot (WB). GTX02530

GTX02530 WB Image

WB analysis of extracts from HUVEC cells using GTX02530 SMO antibody. The lane on the left was treated with the antigen-specific peptide.

Anti-Smoothened homolog antibody used in IHC (Paraffin sections) (IHC-P). GTX02530

GTX02530 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX02530 Smoothened homolog antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Smoothened homolog antibody used in IHC (Paraffin sections) (IHC-P). GTX02530

GTX02530 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX02530 Smoothened homolog antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Smoothened homolog antibody used in IHC (Paraffin sections) (IHC-P). GTX02530

GTX02530 IHC-P Image

IHC-P analysis of rat skin tissue using GTX02530 Smoothened homolog antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

REFERENCE

Application Reference

Natalia Garcia et al. Reprod Sci 2022; 29 (3) : 781-790 Evaluation of Hedgehog Pathway Inhibitors as a Therapeutic Option for Uterine Leiomyosarcoma Using the Xenograft Model.
Application : IHC-P
Reactivity : Human
Submit a Reference

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Package List Price ($)
$ 399