The small regulatory GTPase ARL13B is expressed by most primary cilia and is considered to be a marker for these structures. It is involved in many cellular processes (e.g., cilia biogenesis and maintenance, axon guidance, Hedgehog (HH) signaling, olfactory sensory neuron maturation) and plays an evolutionarily conserved role mediating cilia function in multiple organs (1). ARL13B-null mice are embryonic lethal while human missense mutations in ARL13B cause the ciliopathy Joubert syndrome (JS), a multisystem, autosomal recessive disorder characterized by defective primary cilia (1, 2).

GeneTex is proud to offer the newly developed recombinant rabbit monoclonal ARL13B antibody [HL2173] (GTX638177) to detect the human ARL13B protein in western blot and immunocytochemistry. Please see the product images below and visit www.genetex.com for more details.

 

ARL13B antibody [HL2173] (GTX638177)

RMS 13 and RT-4 cell samples demonstrating differential ARL13B expression at the protein (L) and mRNA (R) levels.	RMS 13 cell wildtype (-) and shRNA-knockdown (+) lysates indicate specific ARL13B signal.	ICC/IF staining of RMS 13 cells fixed with MeOH showing ARL13B enrichment in cilia (yellow arrows).

  

References:

1. Development. 2023 Jan 15;150(2):dev201116. doi: 10.1242/dev.201116. Epub 2023 Jan 19.
2. Am J Hum Genet. 2008 Aug;83(2):170-9. doi: 10.1016/j.ajhg.2008.06.023.