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beta Amyloid

29-Apr-2019
beta-Amyloid antibody

Conformation-specific beta-Amyloid antibody for Alzheimer's disease research

 

Alzheimer’s disease (AD) pathogenesis is widely believed to be driven by the production and deposition of neurotoxic β-Amyloid peptide (Aβ) aggregates that form plaques in the brain. The Aβ molecules are generated from Amyloid Precursor Protein (APP) via β- and γ- secretase cleavage to produce Aβ monomers. Most of the Aβ peptides are 40 amino acids in length (Aβ 1-40, Aβ40), with a small percentage containing 42 residues (Aβ 1-42, Aβ42). These additional two C-terminal amino acids in Aβ42 confer a greater tendency to misfold and subsequently aggregate. Thus, Aβ42 is considered to be more neurotoxic than Aβ40.

GeneTex’s “beta-Amyloid (1-42) antibody – Conformation-Specific (GTX134510, Rabbit pAb) and (GTX635160, mouse mAb)” against Aβ 1-42 oligomers can specifically detect amyloid plaques in the AD brain by IHC-P analysis. The superior specificity and sensitivity of GTX134510 and GTX635160 were confirmed by dot blot and side-by-side IHC-P comparison.
Highlighted Products
Citation Support   KOKD Validation Comparable Abs IP/MS Analysis Orthogonal Validation Protein Overexpression
       
  •   Human Aβ (1-42) oligomer immunogen
  •   Validated for dot blot, IHC-P, and ELISA
  •   High affinity and specificity
  •   Low background staining
  •   Detects beta-Amyloid aggregates in human AD brain tissue by IHC-P
  •   Detects beta-Amyloid aggregates in mouse AD model brain tissue by IHC-P

beta-Amyloid (1-42) antibody – Conformation-Specific (GTX134510)

beta Amyloid (1-42) antibody – Conformation Specific_1

Dot blot analysis of beta-Amyloid peptide (1-42) oligomers or monomers, using beta-Amyloid (1-42) antibody –Conformation-Specific (GTX134510).
beta Amyloid (1-42) antibody – Conformation Specific_2

beta-Amyloid (1-42) antibody –Conformation-Specific (GTX134510) detects beta-Amyloid plaques by IHC-P analysis in occipital lobe tissue from a brain with Alzheimer's disease, but not in normal human brain tissue.
beta Amyloid (1-42) antibody – Conformation Specific_3

beta-Amyloid (1-42) antibody –Conformation-Specific (GTX134510) detects beta-Amyloid plaques by IHC-P analysis in hippocampal tissue from a 3xTg-AD transgenic mouse brain.
beta Amyloid (1-42) antibody – Conformation Specific_4

Side-by-side IHC-P comparison of two conformation-specific beta-Amyloid antibodies in occipital lobe of human brains with Alzheimer's disease (1:500 dilution). Only GeneTex’s beta-Amyloid (1-42) antibody –Conformation-Specific (GTX134510) is able to detect beta-Amyloid plaques in the samples.

Beta amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160)

Beta amyloid (1-42) antibody – Conformation Specific antibody [GT622]_1

Dot blot analysis of beta-Amyloid peptide (1-42) oligomers or monomers, using beta Amyloid (1-42) antibody – Conformation Specific [GT622] (GTX635160).
Beta amyloid (1-42) antibody – Conformation Specific antibody [GT622]_2

beta-Amyloid (1-42) antibody – Conformation Specific [GT622] (GTX635160) detects beta-Amyloid plaques by IHC-P analysis in hippocampal tissue from a 3xTg-AD transgenic mouse brain (left) but not in an age-matched healthy mouse brain (right).
Beta amyloid (1-42) antibody – Conformation Specific antibody [GT622]_3

Sandwich ELISA detection of beta-Amyloid (1-42) peptide aggregates using GTX635160 as capture antibody at concentration of 10 µg/mL and GTX134510 as detection antibody at concentration of 0.45 µg/mL.
Beta amyloid (1-42) antibody – Conformation Specific antibody_4[GT622]

beta-Amyloid (1-42) antibody – Conformation Specific [GT622] (GTX635160) detects beta-Amyloid plaques by IHC-P analysis in cerebellum tissue from a brain with Alzheimer's disease, but not in normal human brain tissue.