PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: All lymphocytes and histiocytes must show a strong membranous CD45 staining.
Negative Control
Tonsil: Squamous epithelial cells must be completely CD45 negative.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human CD45 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Database
Research Area
DATA IMAGES
![Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432](/templates/default/js/lightbox/images/loading.gif "Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432")
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GTX04432 IHC-P Image
IHC-P analysis of human appendix tissue section using GTX04432 CD45 antibody [MSVA-045R] HistoMAX™.
In the appendix, CD45 immunostaining is stronger in lymphocytes than in macrophages. Epithelial cells are completely CD45 negative.
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GTX04432 IHC-P Image
IHC-P analysis of human diffuse large B-cell lymphoma tissue section using GTX04432 CD45 antibody [MSVA-045R] HistoMAX™.
Diffuse large B cell lymphoma showing strong CD45 staining of tumor cells.
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GTX04432 IHC-P Image
IHC-P analysis of human lymph node tissue section using GTX04432 CD45 antibody [MSVA-045R] HistoMAX™.
In the lymph node virtually all hematolymphoid cells are CD45 positive.
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REFERENCE
There are currently no references for CD45 antibody [MSVA-045R] HistoMAX™ (GTX04432). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD45 antibody [MSVA-045R] HistoMAX™ (GTX04432). Be the first to share your experience with this product.
![Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432](/upload/website/prouct_img/small/GTX04432/GTX04432_20230728_IHC-P_22_23072722_931.webp "Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432")
![Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432](/upload/website/prouct_img/small/GTX04432/GTX04432_20230728_IHC-P_152_23072722_861.webp "Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432")
![Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432](/upload/website/prouct_img/small/GTX04432/GTX04432_20230728_IHC-P_282_23072723_403.webp "Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432")
![Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432](/upload/website/prouct_img/normal/GTX04432/GTX04432_20230728_IHC-P_22_23072722_931.webp "Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432")
![Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432](/upload/website/prouct_img/normal/GTX04432/GTX04432_20230728_IHC-P_152_23072722_861.webp "Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432")
![Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432](/upload/website/prouct_img/normal/GTX04432/GTX04432_20230728_IHC-P_282_23072723_403.webp "Anti-CD45 antibody [MSVA-045R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04432")