PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:50-1:100 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A moderate strong membranous and cytoplasmic CTLA4 immunostaining should be seen in a subset of T-lymphocytes with strongest staining in follicular T-helper cells of germinal centres.
Negative Control
Tonsil: The majority of lymphocytes in germinal centres and all epithelial cells should stain negative.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human CTLA4 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytotoxic T-lymphocyte associated protein 4 , ALPS5 , CD , CD152 , CELIAC3 , CTLA-4 , GRD4 , GSE , IDDM12
Cellular Localization
Cell membrane
Background
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439](/templates/default/js/lightbox/images/loading.gif "Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439")
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GTX04439 IHC-P Image
IHC-P analysis of human Hodgkin's lymphoma tissue using GTX04439 CTLA4 antibody [MSVA-152R] HistoMAX™.
Hodgkins lymphoma containing numerous reactive CTLA4 positive lymphocytes.
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GTX04439 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX04439 CTLA4 antibody [MSVA-152R] HistoMAX™.
Normal tonsil with CTLA4 staining of a subset of lymphocytes.
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GTX04439 IHC-P Image
IHC-P analysis of human diffuse large B-cell lymphoma (DLBCL) tissue using GTX04439 CTLA4 antibody [MSVA-152R] HistoMAX™.
Diffuse large B cell lymphoma containing a large number of CTLA4 positive lymphocytes.
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REFERENCE
There are currently no references for CTLA4 antibody [MSVA-152R] HistoMAX™ (GTX04439). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CTLA4 antibody [MSVA-152R] HistoMAX™ (GTX04439). Be the first to share your experience with this product.
![Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439](/upload/website/prouct_img/small/GTX04439/GTX04439_20230728_IHC-P_30_23072722_842.webp "Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439")
![Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439](/upload/website/prouct_img/small/GTX04439/GTX04439_20230728_IHC-P_160_23072722_785.webp "Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439")
![Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439](/upload/website/prouct_img/small/GTX04439/GTX04439_20230728_IHC-P_290_23072723_389.webp "Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439")
![Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439](/upload/website/prouct_img/normal/GTX04439/GTX04439_20230728_IHC-P_30_23072722_842.webp "Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439")
![Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439](/upload/website/prouct_img/normal/GTX04439/GTX04439_20230728_IHC-P_160_23072722_785.webp "Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439")
![Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439](/upload/website/prouct_img/normal/GTX04439/GTX04439_20230728_IHC-P_290_23072723_389.webp "Anti-CTLA4 antibody [MSVA-152R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04439")