PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Positive Control
Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen.
Negative Control
Colon: Collagen IV immunostaining must be absent in epithelial cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified human placental extract
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
collagen type IV alpha 1 chain , collagen type IV alpha 2 chain , collagen type IV alpha 3 chain , collagen type IV alpha 4 chain , collagen type IV alpha 5 chain , collagen type IV alpha 6 chain , BSVD , RATOR , ICH , POREN2 , ATS2 , ATS3 , ATS2 , BFH , CA44 , ASLN , ATS , ATS1 , CA54 , CXDELq22.3 , DELXq22.3 , DFNX6
Database
Research Area
DATA IMAGES
![Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434](/templates/default/js/lightbox/images/loading.gif "Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434")
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GTX04434 IHC-P Image
IHC-P analysis of human lamina muscularis mucosae from stomach tissue using GTX04434 Collagen IV antibody [MSVA-704R] HistoMAX™.
In the intestine collagen IV immunostaining involves basement membranes vessels of all sizes and smooth muscle cells of the lamina muscularis mucosae.
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GTX04434 IHC-P Image
IHC-P analysis of human papillary renal cell carcinoma (PRCC) from kidney tissue using GTX04434 Collagen IV antibody [MSVA-704R] HistoMAX™.
Papillary renal cell carcinoma showing a delicate collagen IV positive membrane between tumor cells and the stroma and an intense staining of vessels.
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GTX04434 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX04434 Collagen IV antibody [MSVA-704R] HistoMAX™.
Collagen IV positive membranes surround pancreatic acini. Small blood vessels do also stain positive.
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REFERENCE
There are currently no references for Collagen IV antibody [MSVA-704R] HistoMAX™ (GTX04434). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Collagen IV antibody [MSVA-704R] HistoMAX™ (GTX04434). Be the first to share your experience with this product.
![Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434](/upload/website/prouct_img/small/GTX04434/GTX04434_20230728_IHC-P_288_23072723_748.webp "Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434")
![Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434](/upload/website/prouct_img/small/GTX04434/GTX04434_20230728_IHC-P_28_23072722_752.webp "Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434")
![Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434](/upload/website/prouct_img/small/GTX04434/GTX04434_20230728_IHC-P_158_23072722_275.webp "Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434")
![Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434](/upload/website/prouct_img/normal/GTX04434/GTX04434_20230728_IHC-P_288_23072723_748.webp "Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434")
![Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434](/upload/website/prouct_img/normal/GTX04434/GTX04434_20230728_IHC-P_28_23072722_752.webp "Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434")
![Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434](/upload/website/prouct_img/normal/GTX04434/GTX04434_20230728_IHC-P_158_23072722_275.webp "Anti-Collagen IV antibody [MSVA-704R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04434")