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Cytokeratin 19 antibody [MSVA-119M] HistoMAX™

HistoMAX
Anti-Cytokeratin 19 antibody [MSVA-119M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04378
Anti-Cytokeratin 19 antibody [MSVA-119M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04378
Anti-Cytokeratin 19 antibody [MSVA-119M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04378

Cat. No. GTX04378

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MSVA-119M

Isotype

 IgG2a

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($519),
100 μl ($299)
See all Cytokeratin 19 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 7.8 is optimal but pH 6.0 results in only slightly weaker stainings. At pH 9.0, the immunostaining is markedly reduced.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Colon: A strong immunostaining is seen in all epithelial cells. Tonsil: A moderate to strong immunostaining occurs in the basal cell layer of the squamous epithelium of the tonsil surface.

Negative Control

Colon: All non-epithelial cells are CK19 negative. Tonsil: All non-basal squamous epithelial cells and all non-epithelial cells are CK19 negative.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human KRT19 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 19 , CK19 , K19 , K1CS

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 19 antibody [MSVA-119M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04378

GTX04378 IHC-P Image

IHC-P analysis of human appendix mucosa tissue section using GTX04378 Cytokeratin 19 antibody [MSVA-119M] HistoMAX™.

Anti-Cytokeratin 19 antibody [MSVA-119M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04378

GTX04378 IHC-P Image

IHC-P analysis of human malignant mesothelioma tissue section using GTX04378 Cytokeratin 19 antibody [MSVA-119M] HistoMAX™.
Malignant mesothelioma with strong cytokeratin 19 positivity.

Anti-Cytokeratin 19 antibody [MSVA-119M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04378

GTX04378 IHC-P Image

IHC-P analysis of human ectocervix tissue section using GTX04378 Cytokeratin 19 antibody [MSVA-119M] HistoMAX™.
In non keratinizing squamous epithelium the basal cells are CK19 positive while the remaining layers are usually negative.

REFERENCE

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REVIEW

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Package List Price ($)
$ 519
$ 299