PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
|
Not tested in other applications.
Calculated MW
Positive Control
In the liver, at least a moderate membranous staining should be seen in hepatocytes while bile duct epithelia stain strongly. In the colon, only epithelium should display a strong membranous staining reaction.
Negative Control
Tonsil or appendix: No staining reaction must be seen in stromal cells such as lymphocytes, plasma cells, smooth muscle cells or endothelial cells.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human E-Cadherin protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO
Cellular Localization
Cell junction , Cell membrane , Endosome
Background
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
Database
Research Area
DATA IMAGES
![Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367](/templates/default/js/lightbox/images/loading.gif "Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367")
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GTX04367 IHC-P Image
IHC-P analysis of human Breast tissue section using GTX04367 E-Cadherin antibody [MSVA-035R] HistoMAX™.
Strong membranous E cadherin staining in a breast cancer NST.
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GTX04367 IHC-P Image
IHC-P analysis of human invasive lobular breast cancer tissue section using GTX04367 E-Cadherin antibody [MSVA-035R] HistoMAX™.
Absence of E cadherin staining in an invasive lobular breast cancer while normal breast glands show strong E cadherin positivity.
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GTX04367 IHC-P Image
IHC-P analysis of human Breast tissue section using GTX04367 E-Cadherin antibody [MSVA-035R] HistoMAX™.
Absence of E cadherin staining in a lobular breast cancer.
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REFERENCE
There are currently no references for E-Cadherin antibody [MSVA-035R] HistoMAX™ (GTX04367). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for E-Cadherin antibody [MSVA-035R] HistoMAX™ (GTX04367). Be the first to share your experience with this product.
![Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367](/upload/website/prouct_img/small/GTX04367/GTX04367_20230728_IHC-P_48_23072722_741.webp "Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367")
![Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367](/upload/website/prouct_img/small/GTX04367/GTX04367_20230728_IHC-P_178_23072723_456.webp "Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367")
![Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367](/upload/website/prouct_img/small/GTX04367/GTX04367_20230728_IHC-P_308_23072723_639.webp "Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367")
![Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367](/upload/website/prouct_img/normal/GTX04367/GTX04367_20230728_IHC-P_48_23072722_741.webp "Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367")
![Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367](/upload/website/prouct_img/normal/GTX04367/GTX04367_20230728_IHC-P_178_23072723_456.webp "Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367")
![Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367](/upload/website/prouct_img/normal/GTX04367/GTX04367_20230728_IHC-P_308_23072723_639.webp "Anti-E-Cadherin antibody [MSVA-035R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04367")