FSD Fluor™ 647 Antibody Labeling Kit

Cat. No. GTX440418
2
Cat. No.  GTX440418
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
GTX440418 Image

FSD Fluor™ 647 Antibody Labeling Kit (GTX440418) was used in flow cytometry analysis.
Sample: non-fixed and non-permeabilized HCT-116 cells.
Left: HCT-116 cells alone.
Right: EpCAM antibody [HL1339] antibody (GTX636759) labelled with FSD Fluor™ 647, dilution: 1:50.

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FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
GTX440418 Image

FSD Fluor™ 647 Antibody Labeling Kit (GTX440418) were used in immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by FSD Fluor™ 647 conjugated-EpCAM antibody [HL1339] (GTX636759) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

2 / 7
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
GTX440418 Image

FSD Fluor™ 647 Antibody Labeling Kit (GTX440418) was used in flow cytometry analysis.
Sample: non-fixed and non-permeabilized HCT-116 cells.
Left: HCT-116 cells alone.
Right: EpCAM antibody [HL1339] antibody (GTX636759) labelled with FSD Fluor™ 647, dilution: 1:50.

3 / 7
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
GTX440418 Image
4 / 7
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
GTX440418 Image
5 / 7
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
GTX440418 Image
6 / 7
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
GTX440418 Image
7 / 7
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
FSD Fluor™ 647 Antibody Labeling Kit. GTX440418
Summary
GeneTex FSD Fluor™ antibody labeling kits provide a simple and rapid method to covalently label antibodies with FSD Fluor™ NHS ester fluorescent dyes by way of the ε-amino group of lysines. The protocol requires only 10 minutes of hands-on time and the conjugated antibody is ready to use within one hour. The kits can be used with GeneTex antibodies, recombinant proteins, and biomolecules that are in PBS-only buffer or in PBS buffer with up to 20% glycerol.

 

Properties of FSD Fluor™ Dyes:

 

Dye

Exmax (nm)

Emmax (nm)

 Fluorescent Color
FSD Fluor™ 488 495 519 Green
FSD Fluor™ 594 590 618 Orange
FSD Fluor™ 647 651 667 Far-Red

 

Antibody Requirements for Conjugation :

 

1. Purified antibody

2. Antibody concentration 0.5-1 mg/ml

3. pH 6.5 – 7.5

 

Acceptable Buffer Components :

 

<0.1% BSA

 <20% Glycerol <0.09% Sodium Azide

<0.025% ProClin™ 300

PBS

Sodium Chloride

EDTA

 Borate buffer Sugars

Unacceptable Buffer Components :

 

Tris

Glycine

 Thimerosal

Merthiolate

DTT

  

 

 

Content
The FSD Fluor™ 647 Antibody Labeling Kit is available in two packing sizes. The 3 x 25 μg package contains three vials of FSD Fluor™ 647 labeling reagents, each capable of labeling 25 μg of antibody. The 1 x 100 μg package contains one vial of FSD Fluor™ 647 labeling reagents, capable of labeling 100 μg of antibody.

 

 

Item

Unit & Qty

(3 x 25 µg)

Unit & Qty

(1 x 100 µg)

 Storage Form
FSD Fluor™ 647 dye 3 vials 1 vial -20°C Lyophilized powder
Enhancer (100 µL) 1 vial 1 vial -20°C Liquid
Quencher (100 µL) 1 vial 1 vial -20°C Liquid

 

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