FSD Fluor™ Triple-Color (488/594/647) Antibody Labeling Kit

Name: FSD Fluor™ Triple-Color (488/594/647) Antibody Labeling Kit
Brand: GeneTex
SKU: GTX440440
Price: 169.00 USD
Rating: 4.7 (3 reviews)

Cat. No. GTX440440

GTX440440 Image

FSD Fluor™ Triple color (488/594/647) Antibody Labeling Kit (GTX440440) was used in flow cytometry analysis.
Sample: non-fixed and non-permeabilized HCT-116 cells.
Left: HCT-116 cells alone.
Right: EpCAM antibody [HL1339] antibody (GTX636759) labelled with FSD Fluor™ 647, dilution: 1:50.

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GTX440440 Image

FSD Fluor™ Triple color (488/594/647) Antibody Labeling Kit (GTX440440) was used in direct immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Green: GFAP stained by GFAP antibody [GT25] (GTX641104) labelled with FSD Fluor™ 488 and DyLight® 488, diluted at 1:100.
Orange: alpha Tubulin antibody [HL2162] (GTX638140) labelled with FSD Fluor™ 594, diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX440440 Image

FSD Fluor™ Triple color (488/594/647) Antibody Labeling Kit (GTX440440) was used in direct immunohistochemical analysis.
Sample: Paraffin-embedded mouse striatum.
Green: GFAP stained by GFAP antibody [GT25] (GTX641104) labelled with FSD Fluor™ 488, diluted at 1:100.
Orange: alpha Tubulin antibody [HL2162] (GTX638140) labelled with FSD Fluor™ 594, diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX440440 Image

FSD Fluor™ Triple color (488/594/647) Antibody Labeling Kit (GTX440440) was used in immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TOMM20 stained by FSD Fluor™ 488 conjugated-TOMM20 antibody [HL1493] (GTX636967) diluted at 1:250.
Red: alpha Tubulin, a marker, stained by FSD Fluor™ 594 conjugated-alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

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GTX440440 Image

FSD Fluor™ Triple color (488/594/647) Antibody Labeling Kit (GTX440440) was used in immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by FSD Fluor™ 594 conjugated-alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

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Summary

GeneTex FSD Fluor™ antibody labeling kits provide a simple and rapid method to covalently label antibodies with FSD Fluor™ NHS ester fluorescent dyes by way of the ε-amino group of lysines. The protocol requires only 10 minutes of hands-on time and the conjugated antibody is ready to use within one hour. The kits can be used with GeneTex antibodies, recombinant proteins, and biomolecules that are in PBS-only buffer or in PBS buffer with up to 20% glycerol.

Properties of FSD Fluor™ Dyes:

Dye	Ex_max(nm)	Em_max(nm)	Fluorescent Color
FSD Fluor™ 488	495	519	Green
FSD Fluor™ 594	590	618	Orange
FSD Fluor™ 647	651	667	Far-Red

Antibody Requirements for Conjugation :

1. Purified antibody

2. Antibody concentration 0.5-1 mg/ml

3. pH 6.5 – 7.5

Acceptable Buffer Components :

<0.1% BSA	<20% Glycerol	<0.09% Sodium Azide
<0.025% ProClin™ 300	PBS	Sodium Chloride
EDTA	Borate buffer	Sugars

Unacceptable Buffer Components :

Tris	Glycine	Thimerosal
Merthiolate	DTT

Content

The FSD Fluor™ Triple-Color (488/594/647) Antibody Labeling Kit contains three different color labeling reagents, each capable of labeling 25 μg of antibody.

Item	Unit & Qty (3 x 25 µg)	Storage	Form
FSD Fluor™ 488 dye	1 vial	-20°C	Lyophilized powder
FSD Fluor™ 594 dye	1 vial	-20°C	Lyophilized powder
FSD Fluor™ 647 dye	1 vial	-20°C	Lyophilized powder
Enhancer (100 µL)	1 vial	-20°C	Liquid
Quencher (100 µL)	1 vial	-20°C	Liquid