PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
Brain: A strong GLUT1 staining of endothelial cells should be seen.
Negative Control
Brain: GLUT1 staining should be absent in all cells/structures except blood vessels.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human GLUT1 protein (around aa 203-305)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
solute carrier family 2 member 1 , CSE , DYT17 , DYT18 , DYT9 , EIG12 , GLUT , GLUT-1 , GLUT1 , GLUT1DS , HTLVR , PED , SDCHCN
Cellular Localization
Cell membrane; Multi-pass membrane protein , Melanosome
Background
This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]
Database
Research Area
DATA IMAGES
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GTX04469 IHC-P Image
IHC-P analysis of human placenta (amnion and chorion) tissue using GTX04469 GluT1 antibody [MSVA-401R] HistoMAX™. Membranous GluT1 staining is very intense in amnion and chorion cells. Endothelial cells also show a particularly strong staining.
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GTX04469 IHC-P Image
IHC-P analysis of human squamous cell carcinoma (SCC) from skin tissue using GTX04469 GluT1 antibody [MSVA-401R] HistoMAX™. Squamous cell carcinoma showing a strong GluT1 immunostaining of tumor cells.
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GTX04469 IHC-P Image
IHC-P analysis of human cerebrum (grey matter) tissue using GTX04469 GluT1 antibody [MSVA-401R] HistoMAX™. A particularly strong GluT1 staining of endothelial cells is seen in the brain.
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REFERENCE
There are currently no references for GluT1 antibody [MSVA-401R] HistoMAX™ (GTX04469). Be the first to share your publications with this product.
REVIEW
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