PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Liver: A strong GS staining should be seen of centrilobular hepatocytes while staining is weak to moderate in Kupffer cells.
Negative Control
Liver: Periportal hepatocytes must be GS negative in the normal liver.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 50-250) of human GLUL protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59
Cellular Localization
Cytoplasm, cytosol , Microsome , Mitochondrion , Cell membrane
Background
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Database
Research Area
DATA IMAGES
![Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471](/templates/default/js/lightbox/images/loading.gif "Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471")
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GTX04471 IHC-P Image
IHC-P analysis of human liver tissue section using GTX04471 Glutamine synthetase antibody [MSVA-750M] HistoMAX™.
Glutamine Synthetase staining is strong in centrilobular hepatocytes weak to moderate in Kupffer cells but absent in periportal hepatocytes.
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GTX04471 IHC-P Image
IHC-P analysis of human hepatocellular carcinoma tissue section using GTX04471 Glutamine synthetase antibody [MSVA-750M] HistoMAX™.
Hepatocellular carcinoma with excessive Glutamine Synthetase positivity of all tumor cells.
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GTX04471 IHC-P Image
IHC-P analysis of human skin squamous epithelium tissue section using GTX04471 Glutamine synthetase antibody [MSVA-750M] HistoMAX™.
In the skin, a nuclear and cytoplasmic Glutamine Synthetase staining can be seen in the lower third and in the top layers of the squamous epithelium.
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REFERENCE
There are currently no references for Glutamine synthetase antibody [MSVA-750M] HistoMAX™ (GTX04471). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Glutamine synthetase antibody [MSVA-750M] HistoMAX™ (GTX04471). Be the first to share your experience with this product.
![Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471](/upload/website/prouct_img/small/GTX04471/GTX04471_20230728_IHC-P_57_23072722_662.webp "Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471")
![Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471](/upload/website/prouct_img/small/GTX04471/GTX04471_20230728_IHC-P_187_23072722_753.webp "Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471")
![Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471](/upload/website/prouct_img/small/GTX04471/GTX04471_20230728_IHC-P_317_23072723_933.webp "Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471")
![Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471](/upload/website/prouct_img/normal/GTX04471/GTX04471_20230728_IHC-P_57_23072722_662.webp "Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471")
![Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471](/upload/website/prouct_img/normal/GTX04471/GTX04471_20230728_IHC-P_187_23072722_753.webp "Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471")
![Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471](/upload/website/prouct_img/normal/GTX04471/GTX04471_20230728_IHC-P_317_23072723_933.webp "Anti-Glutamine synthetase antibody [MSVA-750M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04471")