PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:50-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Colon: Breast cancers with HER2 amplification should show strong positivity. Placenta: A faint staining should be seen at the surface membrane of the syncytiotrophoblast.
Negative Control
Breast: Normal epithelial cells must not show HER2 immunostaining.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encoding the extracellular domain of human c-erbB2
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1
Cellular Localization
Cell membrane , Cytoplasm, perinuclear region , Nucleus , Cytoplasm
Background
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428](/templates/default/js/lightbox/images/loading.gif "Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428")
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GTX04428 IHC-P Image
IHC-P analysis of human invasive breast cancer of no special type (NST) tissue using GTX04428 Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™.
Breast cancer of no special type NST Her2 / ErbB2.
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GTX04428 IHC-P Image
IHC-P analysis of human breast tissue using GTX04428 Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™.
Her2 / ErbB2 staining is lacking in normal breast in case of appropriate formalin fixation.
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GTX04428 IHC-P Image
IHC-P analysis of human invasive breast cancer of no special type (NST) tissue using GTX04428 Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™.
Breast cancer of no special type NST Her2 / ErbB2.
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REFERENCE
There are currently no references for Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ (GTX04428). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ (GTX04428). Be the first to share your experience with this product.
![Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428](/upload/website/prouct_img/small/GTX04428/GTX04428_20230728_IHC-P_60_23072722_819.webp "Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428")
![Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428](/upload/website/prouct_img/small/GTX04428/GTX04428_20230728_IHC-P_190_23072723_451.webp "Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428")
![Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428](/upload/website/prouct_img/small/GTX04428/GTX04428_20230728_IHC-P_320_23072723_261.webp "Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428")
![Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428](/upload/website/prouct_img/normal/GTX04428/GTX04428_20230728_IHC-P_60_23072722_819.webp "Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428")
![Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428](/upload/website/prouct_img/normal/GTX04428/GTX04428_20230728_IHC-P_190_23072723_451.webp "Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428")
![Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428](/upload/website/prouct_img/normal/GTX04428/GTX04428_20230728_IHC-P_320_23072723_261.webp "Anti-Her2 / ErbB2 antibody [MSVA-340R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04428")