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Iba1 antibody [MSVA-955M] HistoMAX™

Orthogonal HistoMAX
Anti-Iba1 antibody [MSVA-955M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04465
Anti-Iba1 antibody [MSVA-955M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04465
Anti-Iba1 antibody [MSVA-955M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04465

Cat. No. GTX04465

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MSVA-955M

Isotype

 IgG2c

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($629),
100 μl ($299)
See all Iba1 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

Colon: An at least moderate to strong cytoplasmic AIF1 immunostaining should be seen in mucosal macrophages.

Negative Control

Colon: AIF1 immunostaining should be absent in all non-inflammatory cells.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human AIF1 protein (around aa 1-146)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Iba1 antibody [MSVA-955M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04465

GTX04465 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX04465 Iba1 antibody [MSVA-955M] HistoMAX™.
Early Iba1 expression is particularly strong in macrophages of the placenta.

Anti-Iba1 antibody [MSVA-955M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04465

GTX04465 IHC-P Image

IHC-P analysis of human duodenum mucosa tissue section using GTX04465 Iba1 antibody [MSVA-955M] HistoMAX™.
A strong Iba1 positivity is seen in mucosal macrophages of the duodenum.

Anti-Iba1 antibody [MSVA-955M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04465

GTX04465 IHC-P Image

IHC-P analysis of human malignant mesothelioma epitheloid tissue section using GTX04465 Iba1 antibody [MSVA-955M] HistoMAX™.
Malignant mesothelioma epitheloid subtype containing numerous Iba1 positive inflammatory cells.

REFERENCE

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REVIEW

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Package List Price ($)
$ 629
$ 299