PRODUCT
Summary
This antibody was validated on 76 different Normal Tissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:75-1:150 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 showed a strongest staining pattern. The pH 7.8 and pH 6.0 is acceptable without resulting in a significant reduction of sensitivity.
|
Not tested in other applications.
Calculated MW
Positive Control
Kidney: Distal tubuli and collecting ducts must show a strong cytoplasmic MUC1 staining while the staining should be at least moderate in the parietal and visceral sheets of the Bowman’s capsule and largely absent in proximal tubuli.
Negative Control
Colon: MUC1 staining should be very weak or absent in colonic epithelium.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human MUC1 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mucin 1, cell surface associated , ADMCKD , ADMCKD1 , CA 15-3 , CD227 , EMA , H23AG , KL-6 , MAM6 , MCD , MCKD , MCKD1 , MUC-1 , MUC-1/SEC , MUC-1/X , MUC1/ZD , PEM , PEMT , PUM
Cellular Localization
Apical cell membrane , Secreted , Cytoplasm , Nucleus
Background
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
![Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397](/templates/default/js/lightbox/images/loading.gif "Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397")
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GTX04397 IHC-P Image
IHC-P analysis of human kidney cortex tissue section using GTX04397 MUC1 antibody [MSVA-672R] HistoMAX.
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GTX04397 IHC-P Image
IHC-P analysis of human malignant mesothelioma epitheloid tissue section using GTX04397 MUC1 antibody [MSVA-672R] HistoMAX.
Malignant mesothelioma epitheloid variant with strong predominantly membranous MUC1 immunostaining of tumor cells.
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GTX04397 IHC-P Image
IHC-P analysis of human pancreas tissue section using GTX04397 MUC1 antibody [MSVA-672R] HistoMAX.
Pancreatic acinar cells show a strong MUC1 immunostaining of the apical membranes and of granular Golgi like structures below the apical surface.
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REFERENCE
There are currently no references for MUC1 antibody [MSVA-672R] HistoMAX (GTX04397). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MUC1 antibody [MSVA-672R] HistoMAX (GTX04397). Be the first to share your experience with this product.
![Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397](/upload/website/prouct_img/small/GTX04397/GTX04397_20230728_IHC-P_79_23072722_238.webp "Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397")
![Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397](/upload/website/prouct_img/small/GTX04397/GTX04397_20230728_IHC-P_209_23072723_691.webp "Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397")
![Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397](/upload/website/prouct_img/small/GTX04397/GTX04397_20230728_IHC-P_339_23072723_282.webp "Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397")
![Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397](/upload/website/prouct_img/normal/GTX04397/GTX04397_20230728_IHC-P_79_23072722_238.webp "Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397")
![Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397](/upload/website/prouct_img/normal/GTX04397/GTX04397_20230728_IHC-P_209_23072723_691.webp "Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397")
![Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397](/upload/website/prouct_img/normal/GTX04397/GTX04397_20230728_IHC-P_339_23072723_282.webp "Anti-MUC1 antibody [MSVA-672R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04397")