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Melan A antibody [MSVA-901M+] HistoMAX™

HistoMAX
Anti-Melan A antibody [MSVA-901M+] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04384
Anti-Melan A antibody [MSVA-901M+] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04384
Anti-Melan A antibody [MSVA-901M+] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04384

Cat. No. GTX04384

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MSVA-901M+

Isotype

 IgG1

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($479),
100 μl ($299)
See all Melan A products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:50-1:100

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

Skin: Virtually all melanocytes should show a strong Melan A+ immunostaining including a weak to moderate staining in melanocytic dendrites. Adrenal gland: An at least moderate Melan A+ immunostaining should be seen in adrenocortical cells.

Negative Control

Kidney: Melan A immunostaining should be absent in all cells. Adrenal gland: Melan A+ immunostaining should be absent in all medullary cells.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length hMART-1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

melan-A , MART-1 , MART1

Cellular Localization

Golgi apparatus , Golgi apparatus, trans-Golgi network membrane , Melanosome

Background

Located in endoplasmic reticulum membrane; melanosome; and trans-Golgi network. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-Melan A antibody [MSVA-901M+] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04384

GTX04384 IHC-P Image

IHC-P analysis of human skin Malignant melanoma tissue section using GTX04384 Melan A antibody [MSVA-901M+] HistoMAX™.
Skin Malignant melanoma with strong Melan A positivity in all tumor cells.

Anti-Melan A antibody [MSVA-901M+] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04384

GTX04384 IHC-P Image

IHC-P analysis of human clear cell renal cell carcinoma tissue section using GTX04384 Melan A antibody [MSVA-901M+] HistoMAX™.
Clear cell renal cell carcinoma showing moderate to strong Melan A immunostaining of tumor cells.

Anti-Melan A antibody [MSVA-901M+] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04384

GTX04384 IHC-P Image

IHC-P analysis of human adrenocortical adenoma tissue section using GTX04384 Melan A antibody [MSVA-901M+] HistoMAX™.
Adrenocortical adenoma showing diffuse strong Melan A positivity.

REFERENCE

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REVIEW

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Package List Price ($)
$ 479
$ 299