PRODUCT
Summary
This antibody was validated on 76 different Normal Tissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
|
Not tested in other applications.
Calculated MW
Positive Control
Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.
Negative Control
Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
ecombinant fragment of human Occludin protein (around aa 282-415)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
occludin , BLCPMG , PPP1R115 , PTORCH1
Cellular Localization
Cell membrane , Cell junction, tight junction
Background
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
Database
Research Area
DATA IMAGES
![Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461](/templates/default/js/lightbox/images/loading.gif "Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461")
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GTX04461 IHC-P Image
IHC-P analysis of human clear cell ovarian carcinoma tissue section using GTX04461 Occludin antibody [MSVA-415M] HistoMAX.
Clear cell ovarian carcinoma with moderate occludin staining of tumor cells.
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GTX04461 IHC-P Image
IHC-P analysis of human squamous epithelium tissue section using GTX04461 Occludin antibody [MSVA-415M] HistoMAX.
In squamous epithelium occludin positivity is regularly seen but staining is reduced or absent in basal and superficial cell layers scaled.
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GTX04461 IHC-P Image
IHC-P analysis of human respiratory epithelium tissue section using GTX04461 Occludin antibody [MSVA-415M] HistoMAX.
Membranous occludin immunostaining of respiratory epithelium scaled.
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REFERENCE
There are currently no references for Occludin antibody [MSVA-415M] HistoMAX (GTX04461). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Occludin antibody [MSVA-415M] HistoMAX (GTX04461). Be the first to share your experience with this product.
![Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461](/upload/website/prouct_img/small/GTX04461/GTX04461_20230728_IHC-P_88_23072722_572.webp "Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461")
![Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461](/upload/website/prouct_img/small/GTX04461/GTX04461_20230728_IHC-P_218_23072722_711.webp "Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461")
![Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461](/upload/website/prouct_img/small/GTX04461/GTX04461_20230728_IHC-P_348_23072723_842.webp "Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461")
![Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461](/upload/website/prouct_img/normal/GTX04461/GTX04461_20230728_IHC-P_88_23072722_572.webp "Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461")
![Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461](/upload/website/prouct_img/normal/GTX04461/GTX04461_20230728_IHC-P_218_23072722_711.webp "Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461")
![Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461](/upload/website/prouct_img/normal/GTX04461/GTX04461_20230728_IHC-P_348_23072723_842.webp "Anti-Occludin antibody [MSVA-415M] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04461")