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14-3-3 (phospho Ser) antibody [3i2(4E2)] GTX14126

References 1

Orthogonal

Summary Recognizes peptides/proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA, recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. Weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences.

Summary This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It reacts also specifically with 5-bromouridine (BrU).

Summary Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)