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African Swine Fever Virus

African Swine Fever Virus

African Swine Fever Virus: An ongoing threat to the global swine industry

African Swine Fever Virus (ASFV) is a deadly (95-100% mortality rate) and highly contagious pathogen that primarily infects domestic and wild pigs. Originating in Sub-Saharan Africa, African Swine Fever (ASF) has been reported in more than 50 countries across Africa, Europe, and Asia and has extended into the Caribbean. Although it cannot infect humans, it has had serious economic impact on the swine industry with almost 25% of the global pig population dying of ASF from 2018-2019.

Transmission of ASF occurs through direct contact with infected pigs as well as exposure to their blood, body fluids, feces, and carcasses. Thus far, no effective treatment or efficacious vaccine has been developed. The currently implemented measures to slow the spread of ASF rely heavily on early detection, on-farm biosecurity measures, culling of herds, and movement control. All of these require reliable diagnostic assays to facilitate early warning, prompt intervention, and monitoring of transmission.

Current diagnostic methodologies include conventional and real-time PCR for detecting viral DNA, ELISA and lateral flow tests for detecting ASFV antigens or antibodies, and the more definitive but time-consuming virus isolation and hemadsorption (HAD).

GeneTex offers an expanding catalog of high-quality validated for various applications, including western blot (WB), ICC/IF, IHC-P, ELISA, Sandwich ELISA and Lateral Flow. Please see the highlighted products below or visit www.genetex.com for details.

GeneTex Antibodies for ASFV Research

✔ Validated for multiple applications: WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA
✔ Validated Sandwich ELISA Pairs
✔ Validated Lateral Flow Pairs
✔ Mouse and Rabbit Monoclonal Antibodies

  

Highlighted Products
Recombinant 

 

ASFV p54 antibody [HL1287] (GTX636701) 

 

Lateral flow analysis using ASFV p54 antibody [HL1287] (GTX636701) as capture antibody and ASFV p54 antibody [GT1075] (GTX635691) as detection antibody.

N: Lysis buffer
P: Recombinant ASFV p54 protein

Sandwich ELISA analysis using ASFV p54 antibody [HL1287] (GTX636701) as capture antibody and HRP-conjugated ASFV p54 antibody [HL1286] (GTX636700) as detection antibody.

 

 

ASFV p54 antibody [HL1289] (GTX636703) 

 

Lateral flow analysis using ASFV p54 antibody [HL1289] (GTX636703) as capture antibody and ASFV p54 antibody [GT1075] (GTX635691) as detection antibody. 

N: Lysis buffer
P: Recombinant ASFV p54 protein

Sandwich ELISA analysis using ASFV p54 antibody [HL1287] (GTX636701) as capture antibody and HRP-conjugated ASFV p54 antibody [HL1289] (GTX636703) as detection antibody.

 

 

Highlighted Antibody Products

  Cat. No. Product Name Host & Clonality Applications
GTX636700 ASFV p54 antibody [HL1286] Rb mAb WB, Sandwich ELISA
GTX636701 ASFV p54 antibody [HL1287] Rb mAb WB, Sandwich ELISA, Lateral Flow
GTX636703 ASFV p54 antibody [HL1289] Rb mAb WB, Sandwich ELISA, Lateral Flow
GTX636563 ASFV p54 antibody [HL1219] Rb mAb WB, ELISA, Sandwich ELISA
  GTX635690 ASFV p54 antibody [GT853] Ms mAb WB, ICC/IF, IHC-P, Lateral Flow, Sandwich ELISA
  GTX635691 ASFV p54 antibody [GT1075] Ms mAb WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA

 

 Selected Recombinant Proteins

Cat. No. Product Name Expression System
GTX135175-pro ASFV p54 (ECD) protein, His Tag E. Coli
GTX137950-pro ASFV p54 (ECD) protein, His Tag HEK293 cells
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